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- MCSP12H48
- 2026年05月28日
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- 库存：
10
- 供应商：
研卉生物
- 现货状态：
有货
- 规格：
48只/箱
MCSP12H48 MilliporeMillicell Hanging Cell Culture Insert, PET 3 µm, 12-well, 48/pk
Polyethylene Terephthalate hanging cell culture insert with pore size of 3.0 µm used in a 12-well plate for cell attachment, cell culture, cell differentiation & ICC.
| Description | |
|---|---|
| Catalogue Number | MCSP12H48 |
| Trade Name |
|
| Description | Millicell Hanging Cell Culture Insert, PET 3 µm, 12-well, 48/pk |
| Background Information | With Millicell inserts, attachment or suspension cells can access media from both their apical and basolateral sides. Cell growth, structure, and function more closely mimic what occurs in vivo. In addition, Millicell inserts make it possible to study both sides of the cell monolayer. Millicell inserts are available for 24-, 12-, or 6-well plates. The inserts are easily prepared for SEM and TEM visualizing techniques, and they are compatible with cellular and/or fluorescent stains. Insert Formats: Millicell Hanging Inserts: Preloaded in receivers or available standalone - For co-culturing and permeability assays - Unique design allows easier basolateral access than other hanging inserts with less risk of contamination - Available in 5 pore sizes and 3 diameters, including a 1 µm pore size that is optically transparent for better visualization by microscopy - NEW: Available preloaded in 24-well receiver plates or as individual inserts in 3 diameters Millicell Standing Inserts: - Promotes excellent cell growth and provides an exceptional opportunity for cell studies - Available with Biopore (PTFE) membrane, MF-Millipore (mixed cellulose esters) membrane, and polycarbonate membrane Millicell Organotypic Insert: - For high cell viability and superior study of three dimensional explant structure - Lower height allows them to fit inside a standard petri dish - The Biopore™ (PTFE) membrane provides high viability—for as long as 40 days—and excellent trans-membrane oxygen transport - The membrane is optically clear and optimized for long-term organotypic explant maintenance Membrane Types: Biopore Membrane (hydrophilic PTFE) - For low protein-binding, live cell viewing, and immunofluorescent applications MF-Millipore™ Membrane (mixed cellulose esters) - For exceptional anatomical and functional polarization Isopore™ Membrane (polycarbonate) - For growth of attachment-dependent cells without matrix PET Membrane (polyethylene terephthalate) - For growth of attachment-dependent cells without matrix With Millicell® inserts, adherent or suspension cells can access media from both their apical and basolateral sides. Cell growth, structure, and function more closely mimic what occurs in vivo. In addition, Millicell® inserts make it possible to study both sides of the cell monolayer. Applications Cell Attachment, Cell Growth, Cell Differentiation , Immunocytochemistry |
| Product Information | |
|---|---|
| Device Configuration | 12-well plate |
| Filter Code | PET |
| Maximum Operating Temperature | 50 °C |
| Number of Wells | 12 |
| Applications | |
|---|---|
| Application | Polyethylene Terephthalate hanging cell culture insert with pore size of 3.0 µm used in a 12-well plate for cell attachment, cell culture, cell differentiation & ICC. |
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文献和实验逐渐凝固成胶状，不可逆。同样的东西在 sigma 叫 ECM。如果购买的小室是已经铺好基质胶的，那么 Matrigel 就不需要购买了。05 下层培养液下层常用含 5%－10% FBS 的培养基，具体浓度根据细胞侵袭力而定，侵袭力弱的细胞可适当提高 FBS 浓度。下层也可用趋化因子，也可将将纤维粘连蛋白加入下层培养液作为趋化因子，但我认为 FBS 仍是最合适的。06 细胞培养板常用于 Transwell 侵袭实验的细胞培养板有6 孔板、12 孔板、24 孔板等，以 24 孔最常用。需注意，细胞培养板
悬空接触下层培养液，无死角、不磨损膜面，换液便捷，适配6孔、12孔、24孔、48孔常规培养板，通用性最强。 2. 站立式细胞小室：底部带有支撑脚，可直接放置于培养孔底部，稳定性更强，适合长期静置培养、高液体体积体系实验，但存在膜底贴合孔板、换液不便的缺点，使用场景相对局限。 （四）按适配板型分类 根据配套培养板规格，可分为6孔、12孔、24孔、48孔细胞小室。孔数越少、单孔面积越大，可接种细胞量越多，适合样本量大、长期培养的实验；24孔、48孔小室通量高、试剂用量少，适合批量筛选、平行
利用 Millicell 培养小室培养皮肤及肺类器官的实验方案
。 在每个胶原蛋白/PHF层上放加入0.5 mL PHKs (4x105细胞/mL细胞悬液)。小心不要破坏胶原蛋白/PHF层，因为这可能会导致渗漏或收缩。在培养小室的外侧加入2ml角质细胞培养液，放入12孔的常规培养皿中。37°C过夜孵育。第二天，在不移除原培养基的情况下，在每个培养小室的内侧添加0.5 mL角质细胞培养基，并在37°C下孵育2天。PHKs应在胶原层上进行三天的液体培养。在第三天液体培养后，高质量的PHKs将接近100%聚合。 第 4 天，从每个小室轻轻中吸出培养基。避免接触
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