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- 库存：
10000
- 现货状态：
大量现货
- 供应商：
杭州欣友生物技术有限公司
- 规格：
个
一、12 孔玻底板（激光共聚焦专用培养板）：
玻底培养皿和玻底培养板主要用于要求放大倍数高、培养器皿底面透光性能好的显微实验，如激光共聚焦显微实验、荧光显微实验和相差显微实验等。玻底培养皿和玻底培养板底面薄，透光性能好， 满足了上述实验的要求。同时玻底培养皿和玻底培养板底面的小孔也减少了实验过程中抗体等试剂的使用量。
Cellvis公司为生物研究人员提供优质廉价的玻底皿和玻底板。Cellvis公司的玻底皿和玻底板底面玻璃使用优质玻片，产品采用无细胞毒性的医用胶水粘合，适用于激光共聚焦等需要高分辨率的细胞显微实验，并且能够耐受长期的细胞培养。
12孔玻底板使用专门的模具生产，产品均一，使用便利。塑料板使用高透明度的USP class VI 聚苯乙烯和医用级的黑色母为原料制成。产品适合贴壁细胞的培养。产品在无尘车间中生产，保证洁净度。
二、产品优势：
- 使用优质玻片，厚度一致性非常高，厚度0.17±0.005mm,特别适合激光共聚焦实验
- 使用USP class VI无细胞毒性的胶水
- 在无尘车间内生产处理和包装
- 通过细胞生物学相关实验的质量检测
- 无热原(<0.5EU/ml)
- Ɣ射线辐照灭菌
三、包装规格
| 产品号 | 品牌 | 规格 |
| P12-1.5H-N | Cellvis | 1.5H号玻片（厚度0.17±0.005mm），单个包装，20个每箱。 |
四、玻底培养皿和玻底培养板使用方法(以35mm皿，10mm孔为例)
- 预平衡：在玻底培养皿加入3ml培养液，在培养箱中放置15分钟。
- 加细胞：吸去培养液，在底孔中加入500ul含细胞的培养液。在培养箱中放置2小时，让细胞沉降贴壁。
- 加培养液：小心加入2-3ml不含细胞的培养液。该步骤用于为细胞提供足够的培养液，同时减少由于水份挥发带来的渗透压的变化。
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*根据实验的要求，可以合并步骤2和3，在预平衡后直接加入2-3毫升含细胞的培养液
五、产品尺寸图
六、该产品被引用文献
Norovirus infection results in assembly of virus-specific G3BP1 granules and evasion of eIF2α signaling
https://www.biorxiv.org/content/10.1101/490318v1
Superresolved and reference-free microparticle traction force microscopy (MP-TFM) reveals the complexity of the mechanical interaction in phagocytosis https://www.biorxiv.org/content/10.1101/431221v2
七、企业资质
八、知名合作单位
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文献和实验- Superresolved microparticle traction force microscopy reveals subcellular force patterns in immune cell-target interactions
Daan Vorselen, et al., bioRxiv - Biophysics 2019
Quote: ... cells were transferred to 12-well glass bottom plates (Cellvis, P12-1.5H-N) (1.5×105 cells/well) ... - Loss of adipocyte identity through synergistic repression of PPARγ by TGF-β and mechanical stress
Ewa Bielczyk-Maczyńska, et al., bioRxiv - Cell Biology 2019
Quote: Polyacrylamide gels were prepared in 12-well glass-bottom plates (Cellvis, P12-1.5H-N), which were activated through consecutive incubation with 2% 3-aminopropyltrimethoxysilane (Acros Organics ... - Alternative Cdc20 translational isoforms bypass the spindle assembly checkpoint to control mitotic arrest duration
Mary Jane Tsang, Iain M. Cheeseman, bioRxiv - Cell Biology 2021
Quote: Cells were first seeded in 12-well polymer-bottomed plates (Cellvis, P12-1.5P) and treated as indicated in the figure legends ... - Listeria monocytogenes Co-opts Caveolin-Mediated E-cadherin Trafficking and Macropinocytosis for Epithelial Cell-to-Cell Spread
Prathima Radhakrishnan, et al., bioRxiv - Microbiology 2022
Quote: ... Transfected cells were seeded onto a fibronectin-coated 12-well glass-bottom plate (Cellvis; P12-1.5H-N) and the transfection mix was replaced with full media 8 hours post transfection ... - Virus survival in evaporated saliva microdroplets deposited on inanimate surfaces
Aliza Fedorenko, et al., bioRxiv - Microbiology 2020
Quote: ... and a portion of the load was sprayed on a 12-well glass bottom plate (P12-1.5H-N, Cellvis). Each well was sprayed by pressing the spray nozzle twice ... - Listeria monocytogenes Co-opts Caveolin-Mediated E-cadherin Trafficking and Macropinocytosis for Epithelial Cell-to-Cell Spread
Prathima Radhakrishnan, et al., bioRxiv - Microbiology 2022
Quote: WT E-cad and Null E-cad A431D cells seeded onto fibronectin-coated glass bottom plates (Cellvis; P12-1.5H-N) were infected with L ... - Topologically correct synthetic reconstruction of pathogen social behavior found in deep tissue sites
Stacie A. Clark, et al., bioRxiv - Microbiology 2020
Quote: ... 50 μl of droplets were transferred to a well in a 12-well coverslip-bottom plate (Cellvis, CA, USA, #P12-1.5H-N), approximately 2 × 106 BMDMs in RPMI 1640 supplemented with 10% FBS and 2 mM glutamine were added to the well ... - Development of a 3D Bioprinted Airway Smooth Muscle Model for Manipulating Structure and Measuring Contraction
Jeffery O Osagie, et al., bioRxiv - Physiology 2023
Quote: ... Constructs were washed one final time to remove unbound stain before they were transferred to a glass bottomed 12 well plate (Cellvis P12-1.5H-N). One-to-two drops of SlowFade Diamond Antifade Mountant (Invitrogen S36963 ...
Cited publications before 2019 (19)
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TIAR marks nuclear G2/M transition granules and restricts CDK1 activity under replication stress
Vanesa Lafarga, et al., EMBOPress, Volume 20, Issue 1, 2019
Quote: "HeLadox‐YFP‐TIARr cells were plated on a 12‐well glass bottom plate (Cellvis) and imaged at 15‐min intervals for 12 h on a Nikon Ti‐E microscope with an integrated perfect focus system" -
Antioxidant treatment induces reductive stress associated with mitochondrial dysfunction in adipocytes
Eduard Peris, et al., JBC, December 17, 2018
Quote: "Cultured adipocytes grown and differentiated in 12-well glass bottom black plates (In Vitro Scientific, CA, USA)" -
Norovirus infection results in assembly of virus-specific G3BP1 granules and evasion of eIF2α signaling
M Brocard, et al., BioRxiv, December 09, 2018
Quote: "10^5 BV2 GFP-G3BP1/mCherry-eIF3E Neo Puro cells were seeded on 12-well glass-bottom plates (Cellvis) in 2ml microscopy medium (phenol red free DMEM, 4,5g/L glucose, HEPES, L-glutamine" -
Screening of herbal extracts for TLR2-and TLR4-dependent anti-inflammatory effects
A Schink, et al., Plos One, October 11, 2018
Quote: "HeLa-TLR4 dual reporter cells were seeded on 12-well glass bottom plates (Cellvis, Mountain View, USA) at a concentration of 2.0×10 5 cells/ml in 1 ml complete medium per well and incubated for 48 h at 37°C." -
Superresolved and reference-free microparticle traction force microscopy (MP-TFM) reveals the complexity of the mechanical interaction in phagocytosis
Daan Vorselen, et al., BioRxiv, October 09, 2018
Quote: "Phagocytic assays: For phagocytic assays, cells were transferred to 12-well glass bottom plates (Cellvis, P12-1.5H-N) (1.5x105 cells/well)" -
StaPLs: versatile genetically encoded modules for engineering drug-inducible proteins
Conor L. Jacobs, et al., Nature Methods 2018
Quote: "Fluorescence widefield microscopy of live transfected HeLa cells (in 35-mm glass-bottom four chamber dishes; In Vitro Scientific), live or fixed transfected HEK293-TRE3GmCherry stable cells (in plastic 12-well plates (Greiner Bio-One) or glass-bottom 12-well plates (In Vitro Scientific))" -
Rapid in situ assessment of Cu-ion mediated effects and antibacterial efficacy of copper surfaces
M Rosenberg, et al., Nature: Scientific Reports, volume 8, Article number: 8172 (2018)
Quote: "Dueto the leakage of luminescence between wells in transparent plates and high background luminescence of white plates, we selected black plates from Cellvis (P12–1.5HN)" -
Long-term Live-cell Imaging to Assess Cell Fate in Response to Paclitaxel
Amanda F. Bolgioni, et al., Jove, 2018
Quote: "12-well glass bottomed imaging dish, Cellvis P12-1.5H-N" -
Inheritance of OCT4 predetermines fate choice in human embryonic stem cells
SC Wolff, et al., BioRxiv, January 26, 2018
Quote: "Asynchronous H9 OCT4-mCherry cells were plated on 12-well glass bottom plates (Cellvis) in phenol-red free or clear DMEM/F-12 (Gibco) supplemented with mTeSR1 supplement" -
Bisphenol A induces DSB-ATM-p53 signaling leading to cell cycle arrest, senescence, autophagy, stress response, and estrogen release in human fetal lung fibroblasts
Laziyan Mahemuti, et al., Archives of Toxicology, 2017
Quote: "4 × 104 cells/ mL were seeded in 12-well glass bottom culture plates (In Vitro Scientific, Sunnyvale, CA 94086, USA) and grown in EMEM overnight"
也可用辅酶型维生素B 12 、辅酶 B 12 等词来表示。是由 5- 脱氧腺苷基取代氰钴胺素（ CN － B 12 ）中与钴连接的 CN 基而生成的，所以亦称钴胺酰胺辅酶。其中大多数是核苷酸中的碱基为 5 ， 6- 二甲（基）苯并咪唑的二甲（基）苯并咪唑钴酰胺辅酶（ dimeth － ylbenzimidazole cobamide coenzyme ）和钴胺素辅酶，但也有碱基为腺嘌呤或 5- 羟基苯并咪唑所置换的 B 12 辅酶。 B 12 辅酶是 H.Barker 等在研
丁香园myl0605的观点：前几天为自己养的PC12是未分化还是分化型困扰,查了一些资料,现发上来希望能给有同样问题的战友有一点帮助!培养条件:ＰＣ12细胞培养于铺有鼠尾胶原的玻璃培养瓶中,置于ＣＯ2培养箱(37℃,95%空气5%ＣＯ2),培养基选用ＤＭＥＭ(ｐＨ7.4,高糖),加入10%灭活小牛血清及5%灭活马血清。分化前:ＰＣ12细胞在培养基中多呈圆形,直径15～20μｍ,也有椭圆或多角形的,扁平的细胞少见,倾向于聚集成小团簇状(A图)。分化后:加入ＮＧＦ后的24ｈ内细胞停止分裂,长出似
人 apelin 1 2 (AP1 2 ) 酶联免疫 分析（ ELISA ） 试剂 盒使用说明书 本试剂仅供研究使用 目的：本试剂盒用于测定人血清，血浆及相关液体样本中 apelin 12(AP12) 的 含量。 实验原理 ： 本试剂盒应用双抗体夹心法测定 标本 中 人 apelin 1 2 (AP1 2 ) 水平。用纯化的 抗- apelin 1 2 (AP1 2 ) 抗体包被微孔板，制成固相抗体，往包被单抗的微孔中
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