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玻底培养皿和玻底培养板主要用于要求放大倍数高、培养器皿底面透光性能好的显微实验,如激光共聚焦显微实验、荧光显微实验和相差显微实验等。玻底培养皿和玻底培养板底面薄,透光性能好, 满足了上述实验的要求。同时玻底培养皿和玻底培养板底面的小孔也减少了实验过程中抗体等试剂的使用量。
Cellvis公司为生物研究人员提供优质廉价的玻底皿和玻底板。Cellvis公司的玻底皿和玻底板底面玻璃使用优质玻片,产品采用无细胞毒性的医用胶水粘合,适用于激光共聚焦等需要高分辨率的细胞显微实验,并且能够耐受长期的细胞培养。
12孔玻底板使用专门的模具生产,产品均一,使用便利。塑料板使用高透明度的USP class VI 聚苯乙烯和医用级的黑色母为原料制成。产品适合贴壁细胞的培养。产品在无尘车间中生产,保证洁净度。
产品号 | 品牌 | 规格 |
P12-1.5H-N | Cellvis | 1.5H号玻片(厚度0.17±0.005mm),单个包装,20个每箱。 |
*根据实验的要求,可以合并步骤2和3,在预平衡后直接加入2-3毫升含细胞的培养液
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TIAR marks nuclear G2/M transition granules and restricts CDK1 activity under replication stress
Vanesa Lafarga, et al., EMBOPress, Volume 20, Issue 1, 2019
Quote: "HeLadox‐YFP‐TIARr cells were plated on a 12‐well glass bottom plate (Cellvis) and imaged at 15‐min intervals for 12 h on a Nikon Ti‐E microscope with an integrated perfect focus system"
Antioxidant treatment induces reductive stress associated with mitochondrial dysfunction in adipocytes
Eduard Peris, et al., JBC, December 17, 2018
Quote: "Cultured adipocytes grown and differentiated in 12-well glass bottom black plates (In Vitro Scientific, CA, USA)"
Norovirus infection results in assembly of virus-specific G3BP1 granules and evasion of eIF2α signaling
M Brocard, et al., BioRxiv, December 09, 2018
Quote: "10^5 BV2 GFP-G3BP1/mCherry-eIF3E Neo Puro cells were seeded on 12-well glass-bottom plates (Cellvis) in 2ml microscopy medium (phenol red free DMEM, 4,5g/L glucose, HEPES, L-glutamine"
Screening of herbal extracts for TLR2-and TLR4-dependent anti-inflammatory effects
A Schink, et al., Plos One, October 11, 2018
Quote: "HeLa-TLR4 dual reporter cells were seeded on 12-well glass bottom plates (Cellvis, Mountain View, USA) at a concentration of 2.0×10 5 cells/ml in 1 ml complete medium per well and incubated for 48 h at 37°C."
Superresolved and reference-free microparticle traction force microscopy (MP-TFM) reveals the complexity of the mechanical interaction in phagocytosis
Daan Vorselen, et al., BioRxiv, October 09, 2018
Quote: "Phagocytic assays: For phagocytic assays, cells were transferred to 12-well glass bottom plates (Cellvis, P12-1.5H-N) (1.5x105 cells/well)"
StaPLs: versatile genetically encoded modules for engineering drug-inducible proteins
Conor L. Jacobs, et al., Nature Methods 2018
Quote: "Fluorescence widefield microscopy of live transfected HeLa cells (in 35-mm glass-bottom four chamber dishes; In Vitro Scientific), live or fixed transfected HEK293-TRE3GmCherry stable cells (in plastic 12-well plates (Greiner Bio-One) or glass-bottom 12-well plates (In Vitro Scientific))"
Rapid in situ assessment of Cu-ion mediated effects and antibacterial efficacy of copper surfaces
M Rosenberg, et al., Nature: Scientific Reports, volume 8, Article number: 8172 (2018)
Quote: "Dueto the leakage of luminescence between wells in transparent plates and high background luminescence of white plates, we selected black plates from Cellvis (P12–1.5HN)"
Long-term Live-cell Imaging to Assess Cell Fate in Response to Paclitaxel
Amanda F. Bolgioni, et al., Jove, 2018
Quote: "12-well glass bottomed imaging dish, Cellvis P12-1.5H-N"
Inheritance of OCT4 predetermines fate choice in human embryonic stem cells
SC Wolff, et al., BioRxiv, January 26, 2018
Quote: "Asynchronous H9 OCT4-mCherry cells were plated on 12-well glass bottom plates (Cellvis) in phenol-red free or clear DMEM/F-12 (Gibco) supplemented with mTeSR1 supplement"
Bisphenol A induces DSB-ATM-p53 signaling leading to cell cycle arrest, senescence, autophagy, stress response, and estrogen release in human fetal lung fibroblasts
Laziyan Mahemuti, et al., Archives of Toxicology, 2017
Quote: "4 × 104 cells/ mL were seeded in 12-well glass bottom culture plates (In Vitro Scientific, Sunnyvale, CA 94086, USA) and grown in EMEM overnight"