
96 孔玻底板(激光共聚焦专用培养板)
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- 详细信息
- 用户评价
- 文献和实验
- 技术资料
- 库存:
1000
- 保修期:
无
- 现货状态:
大量现货
- 供应商:
杭州欣友生物技术有限公司
- 规格:
个,145元一个
一、96 孔玻底板(激光共聚焦专用培养板):
玻底培养皿和玻底培养板主要用于要求放大倍数高、培养器皿底面透光性能好的显微实验,如激光共聚焦显微实验、荧光显微实验和相差显微实验等。玻底培养皿和玻底培养板底面薄,透光性能好, 满足了上述实验的要求。同时玻底培养皿和玻底培养板底面的小孔也减少了实验过程中抗体等试剂的使用量。
Cellvis公司为生物研究人员提供优质廉价的玻底皿和玻底板。Cellvis公司的玻底皿和玻底板底面玻璃使用优质玻片,产品采用无细胞毒性的医用胶水粘合,适用于激光共聚焦等需要高分辨率的细胞显微实验,并且能够耐受长期的细胞培养。
96孔玻底板使用专门的模具生产,产品均一,使用便利。塑料板使用高透明度的USP class VI 聚苯乙烯和医用级的黑色母为原料制成。产品适合贴壁细胞的培养。产品在无尘车间中生产,保证洁净度。
二、产品优势:
- 使用进口优质玻片,特别适合激光共聚焦实验
- 使用进口USP class VI无细胞毒性的胶水
- 在无尘车间内生产处理和包装
- 通过细胞生物学相关实验的质量检测
- 无热原(<0.5EU/ml)
- Ɣ射线辐照灭菌
三、包装规格
| 产品号 | 品牌 | 规格 |
| P96-1-N | Cellvis | 1号玻片(厚度0.13-0.16mm),单个包装,20个每箱。 |
| P96-0-N | Cellvis | 0号玻片(厚度0.085-0.115mm),单个包装,20个每箱。 |
| P96-1.5H-N | Cellvis | 1.5号高平玻片(厚度0.17±0.005mm),单个包装,20个每箱。 |
四、玻底培养皿和玻底培养板使用方法(以35mm皿,10mm孔为例)
- 预平衡:在玻底培养皿加入3ml培养液,在培养箱中放置15分钟。
- 加细胞:吸去培养液,在底孔中加入500ul含细胞的培养液。在培养箱中放置2小时,让细胞沉降贴壁。
- 加培养液:小心加入2-3ml不含细胞的培养液。该步骤用于为细胞提供足够的培养液,同时减少由于水份挥发带来的渗透压的变化。
*根据实验的要求,可以合并步骤2和3,在预平衡后直接加入2-3毫升含细胞的培养液。
五、产品尺寸图
六、引用文献
Visualization of Calcium Ion Loss from Rotavirus during Cell Entry
https://jvi.asm.org/content/92/24/e01327-18
The AP-1 complex regulates AXL expression and determines sensitivity to PI3Kα inhibition in esophagus and head and neck squamous cell carcinoma
https://www.biorxiv.org/content/10.1101/415752v2
七、企业资质


八、知名合作单位







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文献和实验- iPSC Motor Neurons with Familial ALS Mutations Capture Gene Expression Changes in Postmortem Sporadic ALS Motor Neurons
Aaron Held, et al., bioRxiv - Neuroscience 2022
Quote: ... 96-wells plates (Cellvis P96-1.5H-N) were coated with PEI (Sigma 03880 ... - Nucleation of the destruction complex on the centrosome accelerates degradation of β-catenin and regulates Wnt signal transmission
Ryan S. Lach, et al., bioRxiv - Cell Biology 2022
Quote: ... Glass-bottom culture plates (Cellvis # P96-1.5H-N) were pre-treated with bovine fibronectin (Sigma #F1141 ... - A human antibody that broadly neutralizes betacoronaviruses protects against SARS-CoV-2 by blocking the fusion machinery
Dora Pinto, et al., bioRxiv - Immunology 2021
Quote: ... 96-well glass bottom plates (Cellvis P96-1.5H-N) at a density of 20,000 cells/well ... - Myosin-X and talin modulate integrin activity at filopodia tips
Mitro Miihkinen, et al., bioRxiv - Cell Biology 2021
Quote: 96-well glass-bottom plates (Cellvis, P96-1.5H-N) were first coated with a solution of poly-D-lysine (10 μg/ml in PBS ... - The apparent requirement for protein synthesis during G2 phase is due to checkpoint activation
Sarah Lockhead, et al., bioRxiv - Cell Biology 2019
Quote: ... 96-well glass bottom plate (Cellvis, P96-1.5H-N) the day before microscopy at such a density that they were sub-confluent even at the end of the experiment ... - Cell migration CRISPRi screens in human neutrophils reveal regulators of context-dependent migration and differentiation state
Nathan M. Belliveau, et al., bioRxiv - Cell Biology 2022
Quote: ... glass bottom 96 well plates (Cellvis, #P96-1.5H-N) were treated with 1% BSA (Millipore Sigma ... - Single cell preparations of Mycobacterium tuberculosis damage the mycobacterial envelope and disrupt macrophage interactions
Ekansh Mittal, et al., bioRxiv - Microbiology 2022
Quote: ... black plates (P96-1.5H-N, Cellvis, Mountain View, USA) were coated with 100μL TNF-α capture antibody (2 μg/mL in PBS ... - iPSC Motor Neurons with Familial ALS Mutations Capture Gene Expression Changes in Postmortem Sporadic ALS Motor Neurons
Aaron Held, et al., bioRxiv - Neuroscience 2022
Quote: ... astrocytes were seeded into 96-wells plates (Cellvis P96-1.5H-N) coated with PEI and laminin (see neuron differentiation) ... - The intensities of canonical senescence biomarkers integrate the duration of cell-cycle withdrawal
Humza M. Ashraf, et al., bioRxiv - Systems Biology 2023
Quote: ... 96-well glass-bottom plate (Cellvis Cat. No. P96-1.5H-N) 24h prior to fixation for 15 minutes with 4% PFA in PBS ... - The intensities of canonical senescence biomarkers integrate the duration of cell-cycle withdrawal
Humza M. Ashraf, et al., bioRxiv - Systems Biology 2023
Quote: ... 96-well glass-bottom plate (Cellvis Cat. No. P96-1.5H-N) for live-cell imaging that started the following day ...
Cited publications before 2019 (52)
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Superresolution microscopy with novel BODIPY-based fluorophores
Amy M. Bittel, et al., Plos One, October 26, 2018
Quote: "GLOX oxygen scavenger, redox reagent, and additives were mixed and added to the sample 10–30 min before imaging in a sealed 96 well plate with cover glass bottom (CellVis, Mountain View, CA)." -
Microtubules Gate Tau Condensation to Spatially Regulate Microtubule Functions
Ruensern Tan, et al., BioRxiv, September 22, 2018
Quote: "Continuous imaging assays: Tau condensation assays (Fig. 1A-C, S1A) were conducted in Cellvis 96-well Glass Bottom Plate (Cellvis, #P96-1.5H-N) as previously described" -
Inhibitor experiments Fresh isolated human neutrophils (10,000 per well) were seeded in 96-glassbottom-well-plates (In vitro scientific) and activated for NETosis with PMA, final concentration 100 nM
Elsa Neubert, et al., Nature Communications volume 9, Article number: 3767 (2018)
Quote: "Inhibitor experiments: Fresh isolated human neutrophils (10,000 per well) were seeded in 96-glassbottom-well-plates (In vitro scientific) and activated for NETosis with PMA, final concentration 100 nM" -
A FRET-Based approach to study the SUMOylation of Serotonin 1A Receptors
S Kaur, et al., Ku Schoolworks, 2018
Quote: "or in 96-well glass bottom plates (Catalog no. #P96-1-N, CellVis, Mountain View, CA, USA) at a seeding density of 15,000 cells per well." -
Development of a Novel 3D Tumor-tissue Invasion Model for High-throughput, High-content Phenotypic Drug Screening
T. J. Puls, et al., Nature: Scientific Reports, volume 8, Article number: 13039 (2018)
Quote: "Overall dimensions and post spacing were optimized to accommodate glass-bottom 96-well plates (Cellvis, Mountain View, CA; Supplemental Fig. S1)." -
Parallel High-Resolution Imaging of Leukocyte Chemotaxis Under Agarose with Rho-Family GTPase Biosensors
GRR Bell, et al., Rho GTPases pp 71-85, 2018
Quote: "Imaging Materials. 1. Glass-bottom 96-well plate (Cellvis, Catalog #P96–1.5HN or equivalent)" -
Ki67 is a Graded Rather than a Binary Marker of Proliferation versus Quiescence
I Miller, et al., Cell Reports, Volume 24, Issue 5, 31 July 2018, Pages 1105-1112
Quote: "96 well glass bottom plate, Cellvis, Cat# P96-1.5HN" -
Nile Red derivatives enable improved ratiometric imaging for nerve-specific contrast
Jesse R. Korber, et al., J. of Biomedical Optics, 23(7), 076002 (2018).
Quote: "For imaging studies, cells were grown in 96-well glass bottom plates (Cellvis, Mountain View, California) for 48 h, with a final confluence of <80%." -
Add-on plasmonic patch as a universal fluorescence enhancer
J Luan, et al., Nature: Light: Science & Applicationsvolume 7, Article number: 29 (2018)
Quote: "Fluorescence-linked immunosorbent assay was first implemented using 96-well plates with a glass bottom (Cellvis). The glass surface of each well was treated to achieve aldehyde functionality" -
Giardia's ventral disc is hyperstable and composed of over 80 disc-associated proteins
Christopher Nosala, et al., BioRxiv July 03, 2018.
Quote: "Confocal Microscopy: 3D stacks and time lapse movies were acquired of live cells grown in 96-well #1.5 black glass14 bottom imaging plates (In Vitro Scientific)."
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