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玻底培养皿和玻底培养板主要用于要求放大倍数高、培养器皿底面透光性能好的显微实验,如激光共聚焦显微实验、荧光显微实验和相差显微实验等。玻底培养皿和玻底培养板底面薄,透光性能好, 满足了上述实验的要求。同时玻底培养皿和玻底培养板底面的小孔也减少了实验过程中抗体等试剂的使用量。
Cellvis公司为生物研究人员提供优质廉价的玻底皿和玻底板。Cellvis公司的玻底皿和玻底板底面玻璃使用优质玻片,产品采用无细胞毒性的医用胶水粘合,适用于激光共聚焦等需要高分辨率的细胞显微实验,并且能够耐受长期的细胞培养。
96孔玻底板使用专门的模具生产,产品均一,使用便利。塑料板使用高透明度的USP class VI 聚苯乙烯和医用级的黑色母为原料制成。产品适合贴壁细胞的培养。产品在无尘车间中生产,保证洁净度。
产品号 | 品牌 | 规格 |
P96-1-N | Cellvis | 1号玻片(厚度0.13-0.16mm),单个包装,20个每箱。 |
P96-0-N | Cellvis | 0号玻片(厚度0.085-0.115mm),单个包装,20个每箱。 |
P96-1.5H-N | Cellvis | 1.5号高平玻片(厚度0.17±0.005mm),单个包装,20个每箱。 |
*根据实验的要求,可以合并步骤2和3,在预平衡后直接加入2-3毫升含细胞的培养液。
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Superresolution microscopy with novel BODIPY-based fluorophores
Amy M. Bittel, et al., Plos One, October 26, 2018
Quote: "GLOX oxygen scavenger, redox reagent, and additives were mixed and added to the sample 10–30 min before imaging in a sealed 96 well plate with cover glass bottom (CellVis, Mountain View, CA)."
Microtubules Gate Tau Condensation to Spatially Regulate Microtubule Functions
Ruensern Tan, et al., BioRxiv, September 22, 2018
Quote: "Continuous imaging assays: Tau condensation assays (Fig. 1A-C, S1A) were conducted in Cellvis 96-well Glass Bottom Plate (Cellvis, #P96-1.5H-N) as previously described"
Inhibitor experiments Fresh isolated human neutrophils (10,000 per well) were seeded in 96-glassbottom-well-plates (In vitro scientific) and activated for NETosis with PMA, final concentration 100 nM
Elsa Neubert, et al., Nature Communications volume 9, Article number: 3767 (2018)
Quote: "Inhibitor experiments: Fresh isolated human neutrophils (10,000 per well) were seeded in 96-glassbottom-well-plates (In vitro scientific) and activated for NETosis with PMA, final concentration 100 nM"
A FRET-Based approach to study the SUMOylation of Serotonin 1A Receptors
S Kaur, et al., Ku Schoolworks, 2018
Quote: "or in 96-well glass bottom plates (Catalog no. #P96-1-N, CellVis, Mountain View, CA, USA) at a seeding density of 15,000 cells per well."
Development of a Novel 3D Tumor-tissue Invasion Model for High-throughput, High-content Phenotypic Drug Screening
T. J. Puls, et al., Nature: Scientific Reports, volume 8, Article number: 13039 (2018)
Quote: "Overall dimensions and post spacing were optimized to accommodate glass-bottom 96-well plates (Cellvis, Mountain View, CA; Supplemental Fig. S1)."
Parallel High-Resolution Imaging of Leukocyte Chemotaxis Under Agarose with Rho-Family GTPase Biosensors
GRR Bell, et al., Rho GTPases pp 71-85, 2018
Quote: "Imaging Materials. 1. Glass-bottom 96-well plate (Cellvis, Catalog #P96–1.5HN or equivalent)"
Ki67 is a Graded Rather than a Binary Marker of Proliferation versus Quiescence
I Miller, et al., Cell Reports, Volume 24, Issue 5, 31 July 2018, Pages 1105-1112
Quote: "96 well glass bottom plate, Cellvis, Cat# P96-1.5HN"
Nile Red derivatives enable improved ratiometric imaging for nerve-specific contrast
Jesse R. Korber, et al., J. of Biomedical Optics, 23(7), 076002 (2018).
Quote: "For imaging studies, cells were grown in 96-well glass bottom plates (Cellvis, Mountain View, California) for 48 h, with a final confluence of <80%."
Add-on plasmonic patch as a universal fluorescence enhancer
J Luan, et al., Nature: Light: Science & Applicationsvolume 7, Article number: 29 (2018)
Quote: "Fluorescence-linked immunosorbent assay was first implemented using 96-well plates with a glass bottom (Cellvis). The glass surface of each well was treated to achieve aldehyde functionality"
Giardia's ventral disc is hyperstable and composed of over 80 disc-associated proteins
Christopher Nosala, et al., BioRxiv July 03, 2018.
Quote: "Confocal Microscopy: 3D stacks and time lapse movies were acquired of live cells grown in 96-well #1.5 black glass14 bottom imaging plates (In Vitro Scientific)."