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100 µg
蛋白名称:Secretogranin II蛋白, Secretogranin II protein
蛋白构建:A DNA sequence encoding the human SCG2 (AAH22509.1) (Met1-Met617) was expressed with a C-terminal polyhistidine tag.
表达宿主:HEK293 Cells
蛋白纯度:> 95 % as determined by SDS-PAGE
蛋白活性:0
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Pro 17
蛋白分子量:The recombinant human SCG2 comprises 612 amino acids and has a predicted molecular mass of 70.5 kDa.
蛋白NP号:AAH22509.1
蛋白氨基酸序列:Met1-Met617
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Ubiquitinated protein detection is often troublesome since in most cases this modification reduces the half-life of targeted proteins, inducing their degradation. Furthermore, ubiquitination is reversible thanks to the action of highly
Strep-tag®技术是亲和纯化重组蛋白的常用工具,它基于自然界中最强的非共价相互作用之一,即生物素与链霉亲和素的相互作用。该纯化系统的突出特点是纯化过程温和、目的蛋白纯度高、特异性强、兼容多种表达系统,对于有挑战性蛋白的纯化十分有帮助,因此近些年来脱颖而出。 本文将从以下方面介绍Strep-tag®技术: 标签介绍 纯化原理 标签-配体 纯化步骤 系统特点 标签介绍 目前有2种广泛使用的Strep标签: 它们分别是: Strep-tag®II 8 aa小标签(Trp-Ser
Isolation and Culture of Human Alveolar Type II Pneumocytes
, through which another crucial function, provision of alveolar surfactant, occurs. Surfactant synthesis and secretion is a unique feature of type II cells; surfactant consists of a high proportion of phospholipids (approx. 90%) and a small proportion of protein (approx. 10
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