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- Qiagen
- 70042
- 2025年07月12日
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货号：70042
产品名称：Oligotex mRNA Midi Kit
描述：For 12 mRNA midipreps: 700 ul Oligotex Suspension, Small Spin Columns, Collection Tubes (1.5 ml), RNase-free Reagents and Buffers
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文献和实验Fastfilter Plasmid Midi Kit Spin Protocol
to completely neutralize the solution. Complete neutralization of the solution is vital of obtaining good yields. 6. Add 2 ml Buffer GBT, cover and gently mix by inverting tube 3-4 times. 7. Prepare the HiBind Midi Column. Place a HiBind Midi Column
of containing most of the necessary reagents pre-packaged in RNase-free quality （e.g. polyATtract from Promega or Oligotex mRNA kit from Qiagen work well for Neurospora, Table 1）。 Depending on the kit size, this allows isolation of poly（A）-RNA from 0.25 to 5 mg
Fungal Midi DNA Kit Protocol for Fresh/Frozen Specimens
that may have formed) to a HiBind® DNA Midi-column placed in a 15 mL collection tube (supplied) . Centrifuge the column at 8,000 x g for 5 min to bind DNA. Discard the flow-through liquid and reuse the collection tube. 6. Place column to a same collection tube
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