For 100 x 50ul multiplex PCR reactions: 2x QIAGEN Multiplex PCR Master Mix (HotStarTaq® DNA Polymerase,Multiplex PCR Buffer, dNTP Mix) (providing a final concentration of 3 mM MgCl2, 3 x 0.85 ml), 5x Q-Solution (1 x 2.0 ml), distilled water (2 x 1.7 ml)