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货号:133079
产品名称:NeXtalStock PEG 2,000 MME (200)
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文献和实验Large Scale Plasmid Preps: PEG method
. Resuspend pellet in 500 µL of TE. Add 120 µL 5M NaCl, mix. Add 400 µL of 20% PEG 8000. Incubate on ice for 20 min. Pellet at 14,000 rpm, 10 min. 12. Discard supernatant. Pulse spin and discard remainder of supernatant. Resuspend in 200 µL of TE
centrifuge bottle, centrifuge at 10,000 rpm for 30 minutes at 4deg C in the RC5-B using the GSA rotor. For cesium chloride-gradient purification: 7a. Pool the cleared supernatants into to a clean beaker, add one-fourth volume of 50% PEG/0.5 M NaCl
Purification of Plasmid from 50 ml-culture
the supernatant. Remove all of the fluid using pipetman. 10. Dissolve the pellet in 600 ul of TE. Transfer the solution into a microfuge tube. 11. Add 200 ul of 8M LiCl. Mix well, and then spin the solution at 14,000 rpm for 5 min at 4 C. 12
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