C0080 碘化丙啶PI溶液（1mg/ml）科研用

 碘化丙啶PI溶液（1mg/ml）说明书
货号：C0080
规格：1ml/1ml×10
保存：-20℃，有效期至少2年
产品说明：
碘化丙啶（Propidium Iodide，PI）是一种可对DNA 染色的细胞核染色试剂。它是一种溴化乙啶的类似
物，在嵌入双链DNA 后释放红色荧光。PI 不能穿透完整细胞膜，但对凋亡晚期细胞和死细胞的破损细胞
膜能够穿透，并使细胞核红染。PI 经常被用来与Calcein-AM 或者FDA 等荧光探针一起使用，能同时对活
细胞和死细胞染色。PI-DNA 复合物的激发和发射波长分别为535 nm 和615 nm。常用于流式细胞仪分析和
显微镜观察，检测细胞凋亡(apoptosis)或细胞坏死(necrosis)。
本产品为PI 水溶液，浓度为1mg/ml。使用时根据实验不同直接将本产品用相应溶液稀释到工作
浓度。
使⽤⽅法：（仅供参考）
培养细胞染色
1、取适量PI 水溶液加到PBS 中，制备成10-50 M（6.7-33.4g/ml）的PI 溶液。
2、将1/10 培养基体积的PI 溶液加入到细胞培养基中。
3、在37℃培养细胞10-20 分钟。
4、用PBS 或合适的缓冲液洗细胞两次。
5、置于荧光显微镜下观察，激发波长为535 nm，发射波长为615 nm。
注意事项：
PI 被普遍认为具有致癌性，操作时应戴手套，并避免交叉污染。
本产品需避光，并尽量避免反复冻融。
相关产品：
C0060 DAPI 溶液（1mg/ml）
CA1020 ANNEXIN V-FITC/PI 凋亡检测试剂盒
12100 DMEM(H)培养基
S9000 特级胎牛血清

相关文献：
《Medicinal supplement genipin induces p53 and Bax?dependent apoptosis in colon cancer cells》 作者:Jingwang Ye, Jing Li, Xiangfeng Wang, Ling Li 期刊:Oncology Letters 影响因子:1.871 PMID:30127884
《MicroRNA-145 inhibits proliferation and promotes apoptosis of HepG2 cells by targeting ROCK1 through the ROCK1/NF-κB signaling pathway.》 作者:R.-K. Wang, X.-M. Shao, J.-P. Yang, H.-L. Yan, Y. Shao 期刊:European Review for Medical and Pharmacological Sciences 影响因子:2.721 PMID:31002128
《N-acetylcysteine decreases malignant characteristics of glioblastoma cells by inhibiting Notch2 signaling》 作者:Jie Deng, An-Dong Liu, Guo-Qing Hou, Xi Zhang, Kun Ren, Xuan-Zuo Chen, Shawn S. C. Li, Yao-Song Wu and Xuan Cao 期刊:Journal of Experimental & Clinical Cancer Research 影响因子:5.646 PMID:30606241
《Flavones and flavonols exert cytotoxic effects on a human oesophageal adenocarcinoma cell line (OE33) by causing G2/M arrest and inducing apoptosis》 作者:Qiang Zhang,Xin-Huai Zhao,Zhu-Jun Wang 期刊:Food and Chemical Toxicology 影响因子:3.977 PMID:18331776
《Repair of osteochondral defects in a rabbit model with artificial cartilage particulates derived from cultured collagen-chondrocyte microspheres》 作者:Cheng Yu,Jun Liu,Gonggong Lu,Yuxing Xie,Yong Sun,Qiguang Wang,Jie Liang,Yujiang Fan,and Xingdong Zhang 期刊:Journal of Materials Chemistry B
 影响因子:4.776 PMID:
《Characterization of Lycium barbarum polysaccharide and its effect on human hepatoma cells》 作者:M Zhang, X Tang, F Wang, Q Zhang, Z Zhang 期刊:International journal of Biological Macromolecules 影响因子:1.422 PMID:23817098
《Extracellular-controlled breast cancer cell formation and growth using non-UV patterned hydrogels via optically-induced electrokinetics》 作者:Na Liu, Wenfeng Liang, Lianqing Liu, Yuechao Wang, John D. Mai, Gwo-Bin Lee and  Wen J. Li 期刊:Lab on a Chip 影响因子:5.995 PMID:24531214
《TREM-1 expression in rat corneal epithelium with Aspergillus fumigatus infection》 作者:Li-Ting Hu, Zhao-Dong Du, Gui-Qiu Zhao, Sheng Qiu, Nan Jiang, Jing Lin, Qian Wang, and Qiang Xu 期刊:International Journal of Ophthalmology 影响因子:0.119 PMID:25938031
《Anti-tumor outcome evaluation against non-small cell lung cancer in vitro and in vivo using PolyI:C as nucleic acid therapeutic agent》 作者:Yedan Wu, Wei Huang, Liqing Chen,Mingji Jin, Zhonggao Gao, Changshan An, and Haixiang Lin 期刊:American Journal of Translational Research 影响因子:3.266 PMID:31105808
《Endometrial mesenchymal stem cells isolated from menstrual blood repaired epirubicin-induced damage to human ovarian granulosa cells by inhibiting the expression of Gadd45b in cell cycle pathway》 作者:Zhongrui Yan, Fengyi Guo, Qing Yuan, Yu Shao, Yedan Zhang, Huiyan Wang, Shaohua Hao and Xue Du 期刊:Stem Cell Research & Therapy 影响因子:4.627 PMID:30606243
《Unfolded Protein Response Differentially Regulates TLR4-Induced Cytokine Expression in Distinct Macrophage Populations》 作者:Lei Zhang, Paul G. Pavicic Jr., Shyamasree Datta, Qiaoling Song, Xiaohan Xu, Wei Wei, Fan Su, Patricia A. Rayman, Chenyang Zhao and Thomas Hamilton 期刊:Frontiers in Immunology 影响因子:4.716 PMID:31293572
《Ultrasound-assisted synthesis of chitosan from fungal precursors for biomedical applications》 作者:Li-Fang Zhu,Jing-Song Li,John Mai,Ming-Wei Chang 期刊:Chemical Engineering Journal 影响因子:8.355 PMID:

Solarbio
Beijing Solarbio Science & Technology Co., Ltd
Tel: 010-56371210
Fax: 010-56371281/82
Http://www.solarbio.cn