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- 德国
- 12032937001
- 2025年10月30日
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
见产品外包装
- 英文名：
FastStart™ Taq DNA Polymerase
- 库存：
需确认
- 供应商：
嵘崴达
- 保存条件：
−20°C
- 规格：
500 reactions
FastStart™ Taq DNA聚合酶，5 U / μl
FastStart™ Taq DNA Polymerase, 5 U/μl
| packaging | pkg of 1,000 U (12032937001 [4x250 U]) |
| pkg of 2,500 U (12032945001 [10x250 U]) | |
| pkg of 5,000 U (12032953001 [20x250 U]) | |
| pkg of 100 U (12032902001) | |
| pkg of 500 U (12032929001 [2x250 U]) |
General description
FastStart™ Taq DNA Polymerase is a versatile enzyme that can be used in a wide variety of applications and on multiple instrument platforms. This modified, thermostable recombinant Taq DNA polymerase is inactive at temperatures below +75 °C, but is activated by a 2 to 4 minute heat activation step at +95 °C. Since it is inactive at low temperatures, FastStart™ Taq DNA Polymerase cannot elongate non-specific primer-template hybrids that may form at those temperatures. FastStart™ Taq DNA Polymerase is an ideal tool for hot start PCR, because the enzyme remains inactive during PCR set-up and prior to the initial denaturation step.
Application
FastStart™ Taq DNA Polymerase is a thermostable, chemically modified form of recombinant Taq DNA polymerase. The enzyme is inactive at +15 to +25°C during PCR setup, and then activated at +95°C during initial denaturation. This enzyme delivers superior results due to its unique enzyme design and optimized buffer system. FastStart Taq DNA Polymerase is an ideal tool for hot start PCR, because the enzyme remains inactive during PCR set-up and prior to the initial denaturation step.
罗氏试剂
| 12032937001 | FastStart Taq DNA Polymerase, DNA聚合酶 | 1,000 U (4 x 250 U) |
| 10127558001 | ADH, susp. 1g (34ml) | 1 g (34 ml) |
| 10837016001 | Formate Dehydrogenase, 250 u甲酸脱氢酶 | 250 U |
| 11636103001 | PCR Master | 1 kit (for 200 PCR reactions of 50 µl final reaction volume) |
| 11411446001 | Isopropyl-b-D-thiogalactoside | 5 g |
| 11666703001 | PREMIX 10XTBE BUF/WAS 100957 4 | 4 l |
| 10128163001 | Pyruvate Kinase (PK), susp., 1 | 100 mg (10 ml) |
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文献和实验应用的原因.Taq DNA聚合酶还具有逆转录活性， 其作用于类似逆转录酶.此活性温度一般为65～68℃，有Mn2+存在时，其逆转录活性 更高. (二)离子依赖性 aqDNA聚合酶是Mg2+依赖性酶，该酶的催化活性对Mg2+浓度非常敏感.以活性程度 很低的鲑鱼精子DNA为模板，dNTP的浓度为0.7～0.8mmol/L时，用不同浓度Mg2+进行 PCR反应10min，测定结果为Mgcl2浓度在2.0mmol/L时该酶催化活性最高，此浓度能最 大限度地激活TaqDNA聚合酶的活性，Mg
Clonality - X Chromosome Inactivation Assay
) Forward and reverse primers, each 20 µM (Human Androgen Receptor Gene (HUMARA) CAG trinucleotide repeat microsatellite primers are described in Allen et al) AmpliTaq Gold, 5 U/ml (Perkin Elmer) a 32P-dCTP, 20 µCi/µl (NEN) Gel-Mix 6 (Life Technologies
红细胞数的均数有无差别？ H0: μ=μ0 H1: μ≠μ0 α=0.05 今x1=4.660×1012/L,s1=0.575×1012/L,n1=360； x2=4.1781012/L,s2=0。2911012/L，n2=255. 算得的u=13.63＞2.58,P＜0.01,按 α=0.05检验水准拒绝H0，接受H1，可认为该地男女红细胞数的均数不同，男性高于女性。 （二）t检验 可用于两
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