FastStart™ Taq DNA Polymerase is a thermostable, chemically modified form of recombinant Taq DNA polymerase. The enzyme is inactive at +15 to +25°C during PCR setup, and then activated at +95°C during initial denaturation. This enzyme delivers superior results due to its unique enzyme design and optimized buffer system. FastStart Taq DNA Polymerase is an ideal tool for hot start PCR, because the enzyme remains inactive during PCR set-up and prior to the initial denaturation step. It can be applied for: • PCR • Multiplex PCR • Difficult templates e.g., secondary structures or GC-rich sequences • Automated PCR e.g., handling at room temperatures • Hot Start PCR up to 3kb • Hot Start RT-PCR up to 3kb • Quantitative reverse transcription PCR (RT-qPCR) • Bisulfite-specific PCR
Use FastStart™ Taq DNA Polymerase, dNTPack with ready-to-use PCR nucleotide mix.For maximum convenience, select the 2× concentrated ready-to-use FastStart™ PCR Master.
Features and Benefits
FastStart Taq DNA Polymerase is a modified recombinant Taq DNA Polymerase, inactive at temperatures below +75°C. The kit includes an optimized PCR buffer and GC-RICH Solution for handling a wide range of templates. High enzyme stability enables pipetting by robotic stations. • Higher specificity, sensitivity, and yield: Hot start PCR makes PCR setup easier. • Use robotic setup. Use this enyzme mix stable for 24hours at +15 to +25°C. • Prevent PCR carryover contamination. Incorporate dUTP and use Uracil-DNA Glycosylase to pretreat PCR master mixes • optimized polymerase chain reaction (PCR) buffer system and a GC-RICH solution for handling wide range of templates • superior results due to its unique enzyme design and optimized buffer system