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- 询价记录
- 文献和实验
- 技术资料
- 保质期:
见产品外包装
- 英文名:
FastStart™ Taq DNA Polymerase, 5 U/μl
- 库存:
需确认
- 供应商:
嵘崴达
- 保存条件:
−20°C
- 规格:
2,500 reactions
FastStart™ Taq DNA聚合酶,5 U / μl
FastStart™ Taq DNA Polymerase, 5 U/μl
Application
FastStart™ Taq DNA Polymerase is a thermostable, chemically modified form of recombinant Taq DNA polymerase. The enzyme is inactive at +15 to +25°C during PCR setup, and then activated at +95°C during initial denaturation. This enzyme delivers superior results due to its unique enzyme design and optimized buffer system. FastStart Taq DNA Polymerase is an ideal tool for hot start PCR, because the enzyme remains inactive during PCR set-up and prior to the initial denaturation step.
It can be applied for:
• PCR
• Multiplex PCR
• Difficult templates e.g., secondary structures or GC-rich sequences
• Automated PCR e.g., handling at room temperatures
• Hot Start PCR up to 3kb
• Hot Start RT-PCR up to 3kb
• Quantitative reverse transcription PCR (RT-qPCR)
• Bisulfite-specific PCR
Use FastStart™ Taq DNA Polymerase, dNTPack with ready-to-use PCR nucleotide mix.For maximum convenience, select the 2× concentrated ready-to-use FastStart™ PCR Master.
Features and Benefits
FastStart Taq DNA Polymerase is a modified recombinant Taq DNA Polymerase, inactive at temperatures below +75°C. The kit includes an optimized PCR buffer and GC-RICH Solution for handling a wide range of templates. High enzyme stability enables pipetting by robotic stations.
• Higher specificity, sensitivity, and yield:
Hot start PCR makes PCR setup easier.
• Use robotic setup.
Use this enyzme mix stable for 24hours at +15 to +25°C.
• Prevent PCR carryover contamination.
Incorporate dUTP and use Uracil-DNA Glycosylase to pretreat PCR master mixes
• optimized polymerase chain reaction (PCR) buffer system and a GC-RICH solution for handling wide range of templates
• superior results due to its unique enzyme design and optimized buffer system
| 12032953001 | FastStart Taq DNA Polymerase, DNA聚合酶 | 5,000 U (20 x 250 U) |
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| 12032945001 | FastStart Taq DNA Polymerase, DNA聚合酶 | 2,500 U (10 x 250 U) |
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文献和实验The Polymerase Chain Reaction (PCR)
and multi-block systems. •Reactions in tubes or 96-well micro-titre plates. So, I Can Just Go Ahead? -Not so fast. -The PCR technique and the use of Taq DNA polymerase in PCR are both patented. -Even academic and public organisations must pay
High Specificity PCR Amplification Using AccuPrime Taq DNA Polymerase
1–200 ng AccuPrime™ Taq DNA Polymerase 0.25 µl 0.5 µl 1 µl Autoclaved distilled water
Polymerase III in vitro Transcription
Polymerase III in vitro Transcription Steve Hahn For the following reactions, use appropriate shielding and dispose of radioactive waste properly! A 20 microliter transcription reaction contains: 4.0 μl 5X Pol III transcription buffer 0.2 μl
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