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- 2025年07月15日
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Neobioscience
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+4
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1 x 100.0 x ml
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文献和实验Immunohistochemistry (IHC) of Thymus Frozen Sections
Materials Cold Acetone (-20ºC) in Coplin jars PBS, BenchKote paper, plastic lids Anti-digoxigenin-HRP diluent: 1% BSA in PBS (store 10 mL aliquots of BSA at -20ºC) Anti-rat-digoxigenin diluent: 5% Milk powder, 10% mouse serum, 10% goat
IHC frozen sections staining protocol
antibody. Glycine will bind free aldehyde groups that would otherwise bind the primary and secondary antibodies, leading to high background. Background due to free aldehyde groups is more likely to occur when the fixative is glutaraldehyde
)Secondary Antibody15mlIHC-P8125SSignalStain® Boost IHC Detection Reagent (HRP, Mouse)Secondary Antibody15mlIHC-P8889SAnti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 594 Conjugate)Secondary Antibody250µlIF-IC F8890SAnti-mouse IgG (H+L), F(ab
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