木瓜蛋白醇 Papain

木瓜蛋白醇

Papain

from Carica papaya

General description

Papain is a cysteine protease of the papain family. It is a single chain protein. The molecular weight of papain is 23 kDa. Papain is composed of two structural domains and a cleft between them, which consists of the active site.

Application

Papain has been used in the digestion of various brain cells.

木瓜蛋白酶可用于蛋白质的完全蛋白水解切割、天然免疫球蛋白的有限水解以及组织解离（与胶原酶、酯酶和胰蛋白酶一起使用）。该酶可溶解内在膜蛋白并水解纯蛋白多糖生成糖肽。添加半胱氨酸（约0.5% [w/v]）对酶活性至关重要。

Biochem/physiol Actions

Papain exhibits proteolytic activity against amide links, amino acid esters, peptides, proteins. It mainly cleaves peptide bonds containing amino acids such as lysine, arginine and residues succeeding phenylalanine. It can catalyze several reactions, such as hydrolysis, transferase action, specificity and acyl-enzyme intermediate.
 

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主要经营的试剂品牌：
Roche试剂，部分常用现货，周三定货，2-3周到货。
GE试剂，耗材，部分常用现货，周二，周五定货，3周左右。
SIGMA试剂，周一，五定货，3周左右到货。
THERMO试剂，部分现货，周三定货，2-3周到货。
ABCAM试剂，国内现货三天左右，期货四周左右。
Peprotech试剂，部分常用现货，周三定货，2-3周到货。

罗氏原料，请来电来邮咨询。

OXOID，BD培养基货期八周左右，部分现货。
MCE激动剂，ACROS、ALFA、阿拉丁等生化试剂现货定购。

 

10108014001	Papain木瓜蛋白酶	100 mg (10 ml)
10102946001	ABTS	2 g
11112589001	Blocking Reagent for ELISA	27 g (for 1 l)
10476986001	Endoproteinase Lys-C赖氨酸	3 U
10791156001	Endoproteinase Glu-C (V8 ProteV8蛋白内切酶Glu-C位点	2 mg
11332473001	Nonidet P40	5 x 10 ml
11112481001	Streptavidin-b-Gal	500 U
11347101001	O-Glycosidase, BSA-freeO-糖苷酶	25 mU