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文献和实验Enrichment of PBMCs with monocytes (The Science Advisory Board)
1. Dilute buffy coat to 150 ml with PBS. 2. Prepare 2 Ficoll (Biocoll) gradients in Leucosep tubes (15 ml Ficoll per tube). 3. Apply 30 ml of diluted blood to each gradient. 4. Centrifuge 30 min at 400 x g without brakes. 5. Collect the PBMC fraction
Immunoprecipitation of metabolically labeled cells
- dilute with Super E to convenient volume; add protease inhibitors, cold Met and antibody. Some antibodies work only after the antigen has been denatured (e.g. Ab 877, Ab 471): to 50 ul of sample in TMT, add 5 µl 10% SDS and incubate at 100 o C
吃核桃补脑，真的靠谱吗？Lancet 子刊告诉你坚持吃核桃六个月，到底会有啥影响
.1016/j.eclinm.2023.101954. 2. Sala-Vila A, Fleming J, Kris-Etherton P, Ros E. Impact of α-linolenic acid, the vegetable ω-3 fatty acid, on cardiovascular disease and cognition. Adv Nutr. 2022;13. 3. Food and Nutrition Board|National Academies. https
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