OBS BOX,LMS PANEL 22, 500pMOL

【转载】protocol for real-time PCR

Mix (2x) 0.5 ml liver cDNA 2 ml primer pair mix (5 pmol/ml each primer) 22.5 ml H2O OR 12.5 ml SYBR Green Mix (2x) 0.2 ml liver cDNA 1 ml primer pair mix (5 pmol/ml each primer) 11.3 ml H2O 4. After PCR is finished, remove the tubes

In Situ Hybridization Using Digoxigenin Labeled Probes

on two 5ml pipettes in a tray lined with moist paper towels. Apply 100µl of a 1:500 dilution of Anti Digoxigenin-POD in 1X PBS, 5mMEDTA. Distribute the solution by placing a 22 x 40 mm coverslip on the slide. 5) Cover the tray with plastic wrap

Lipid analysis－Week 3: GAS LIQUID CHROMATOGRAPHY

4      7.5 min           18:3ME     5     10.2 min           18:2ME    12     10.2 min           18:1ME    60     10.4 min           18:0ME     3     10.8 min           20:0ME     3     14.7 min              22:1ME     5     17.9 min           22:0ME