- 询价
- Biotium
- 美国
- 92244
- 2025年07月09日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20°C
- 保质期：
1年
- 英文名：
Mix-n-Stain Biotin Antibody Labeling Kit, 1x(50-100ug) labeling 92244
- 库存：
99
- 供应商：
Biotium
Mix-n-Stain™ Biotin Antibody Labeling Kits allow you to biotinylate ≤5 ug up to 100 ug of your antibody in just 30 minutes, with minimal hands-on time and no purification.
Features
- Label 5-20 ug, 20-50 ug, or 50-100 ug Ab
- Less than 30 seconds of hands-on time
- 30 minutes total reaction time
- No purification, 100% recovery
- Compatible with BSA, gelatin, ascites
Kit components
- Ultrafiltration spin vial (for antibody concentration or buffer exchange, if needed)
- 10X Mix-n-Stain™ Reaction Buffer
- Lyophilized Reactive Biotin
- Mix-n-Stain™ Storage Buffer
Unrivaled Convenience
Mix-n-Stain™ labeling tolerates common antibody buffer formulations. An ultrafiltration centrifuge vial is provided to quickly remove interfering substances like glycerol, or to concentrate your antibody. With a slight modification in protocol, antibodies can be labeled in the presence of BSA, gelatin, or ascites fluid. The presence of other proteins like BSA or gelatin in the labeling reaction has minimal effect on background fluorescence, because any labeled non-antibody proteins readily wash away during immunofluorescence staining.
Choose the Right Labeling Kit for Your Antibody
Mix-n-Stain™ Antibody Labeling Kits are very simple to use (see the workflow overview above). But before you begin, you must check that your antibody meets the compatibility requirements for labeling, and choose the right labeling protocol. Download the updated and expanded Product Protocol for a labeling check list to help you select the right kit size and labeling protocol for your antibody. Also see our Kit Compatibility and Protocol Selection Flowchart.
We offer Mix-n-Stain™ Kits for labeling antibodies, nanobodies, and small ligands with a wide selection of fluorescent dyes, enzymes, and other labels. See our full selection of Mix-n-Stain™ Antibody Labeling Kits below.
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文献和实验and concentrations will remain consistent for the labeling. B. Add Total volume (50µl,) of Tube 1 (TdT) + remainder of Tube 2 (450µl). Apply 100µl TUNEL reaction mixture (or 100µl Control Label solution for negative control) to each slide.
Revised amino-allyl labeling protocol
and the Howard Hughes Medical Institute http://cmgm.stanford.edu/pbrown/protocols/amino-allyl.htm A. RT Reaction 1. To anneal primer, mix 1-2 m g mRNA with 5 ug of anchored oligo-dT [(dT)20 -VN] (Operon, HPLC purified) in a total volume of 18 m L
DNA labeling by nick translation
Mix (V total = 50 µl): 1 probe mix for N probes NT (10x) 5 µl (N 1) * 5 : b-ME 5 µl
