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- Biotium
- 美国
- 30071-T
- 2025年07月11日
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- 详细信息
- 询价记录
- 技术资料
- 保质期：
＞6个月
- 英文名：
AccuOrange Protein Quantitation Kit
- 库存：
999
- 供应商：
Biotium
AccuOrange™ Protein Quantitation Kit is a highly sensitive fluorescence-based assay for quantitating purified protein samples in 96-well format (excitation/emission = 480/598 nm). The detection range of the assay is 0.1-15 ug/mL protein. AccuOrange is much more sensitive than traditional protein quantitation assays such as BCA, Bradford and Lowry, and shows superior linearity and reproducibility compared to the NanoOrange protein quantitation assay. The assay shows minimal variability between different proteins, and has stable fluorescence signal for up to 16 hours.
AccuOrange is recommended for quantitating purified protein or antibody samples. The tolerance of the AccuOrange assay to salts, buffers, detergents, and other chemicals is shown in Table 1 of the product protocol. The AccuOrange assay has low tolerance for non-ionic detergents, and is not recommended for use with cell lysates containing Triton X-100, sodium deoxycholate, CHAPs, or other non-ionic detergents. The assay can tolerate up to 0.01% SDS (final concentration in assay).The AccuOrange Assay is compatible with the AccuLite™ 470 Mini Fluorometer.
AccuOrange™ Protein Quantitation Kit is a highly sensitive fluorescence-based assay for quantifying purified protein samples in 96-well format.
- Highly sensitive: detect 0.1-15 ug/mL protein
- Excellent linearity and low variability
- Stable fluorescence signal
- Compatible with reducing agents and other additives
- For use with purified protein or antibody samples
- Ex/Em: 480/598 nm
The detection range of the assay is 0.1-15 ug/mL protein. AccuOrange™ is much more sensitive than traditional protein quantitation assays such as BCA, Bradford and Lowry, and shows superior linearity and reproducibility compared to the NanoOrange® protein quantitation assay. The assay shows minimal variability between different proteins, and has stable fluorescence signal for up to 16 hours.
AccuOrange™ is recommended for quantifying purified protein or antibody samples. The tolerance of the AccuOrange™ assay to salts, buffers, detergents, and other chemicals is listed in the product protocol. The AccuOrange™ assay has low tolerance for non-ionic detergents, and is not recommended for use with cell lysates containing Triton™ X-100, sodium deoxycholate, CHAPs, or other non-ionic detergents. The assay can tolerate up to 0.01% SDS (final concentration in assay).
Comparison of AccuOrange™ with other protein quantitation assays
| Assay | Detection Range | Comments |
|---|---|---|
| AccuOrange™ | 0.1-15 ug/mL | Fluorescence-based (480/598 nm) Highly linear Signal stable for at least 16 hours Compatible with reducing agents Not compatible with detergents |
| NanoOrange® | 0.1-10 ug/mL | Fluorescence-based (470/570 nm) Non-linear Fluorescence stable for 6 hours Compatible with reducing agents Not compatible with detergents |
| Modified Lowry | 1-1500 ug/mL | Absorbance-based (750 nm) Non-linear Not compatible with reducing agents or detergents |
| BCA | 20-2000 ug/mL | Absorbance-based (562 nm) Highly linear Signal not stable over time Not compatible with reducing agents Compatible with detergents |
| Bradford (Coomassie®) | 50-500 ug/mL | Absorbance-based (595 nm) Signal not stable over time Non-linear Compatible with reducing agents Not compatible with detergents |
| Pierce® 660 nm | 50-2000 ug/mL | Absorbance-based (660 nm) Non-linear Compatible with reducing agents & detergents |
| A280 | 50-2000 ug/mL | Absorbance-based (280 nm) High protein-protein variability Contaminants and detergents can affect results |
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