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- Biotium
- 美国
- 40005
- 2025年06月30日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20°C
- 保质期：
6个月
- 英文名：
CF568-dUTP 40005
- 库存：
99
- 供应商：
Biotium
CF® dye-dUTP can be used for TUNEL assay, microarray, or to synthesize labeled DNA probes for in situ hybridization and nucleic acid blotting applications.
- Synthesize exceptionally bright and photostable fluorescent DNA probes
- Detect by fluorescence microscopy, flow cytometry, or in gel
- Compatible with fixed cells or tissue sections
- Choice of 8 CF® dye colors from blue to near infrared
- Biotium’s CF® dyes have superior brightness, photostability, and are water-soluble
Supplied in lyophilized form. For PCR applications, Taq polymerase should be used with dUTP conjugates, because dUTP inhibits archaeal polymerases such as Pfu and Vent®. Also see our CF® Dye dCTP Conjugates and TUNEL assay kits, available with a wide selection of bright and photostable CF® dyes.
Superior CF® Dyes
Biotium’s next-generation CF® dyes were designed to be highly water-soluble with advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes. Learn more about CF® Dyes.
dUTP CF® Dye Conjugates
| Product | Dye | Ex/Em | Size | SKU | Purchase |
|---|---|---|---|---|---|
| CF®405S dUTP1 | CF®405S | 404/431 nm | 5 nmol | 40004-T | Purchase 40004-T |
| 25 nmol | 40004 | Purchase 40004 | |||
| CF®405M dUTP | CF®405M | 408/452 nm | 5 nmol | 40100-T | Purchase 40100-T |
| 25 nmol | 40100 | Purchase 40100 | |||
| CF®488A dUTP | CF®488A | 490/515 nm | 5 nmol | 40008-T | Purchase 40008-T |
| 25 nmol | 40008 | Purchase 40008 | |||
| CF®543 dUTP | CF®543 | 541/560 nm | 5 nmol | 40002-T | Purchase 40002-T |
| 25 nmol | 40002 | Purchase 40002 | |||
| CF®568 dUTP | CF®568 | 562/583 nm | 5 nmol | 40005-T | Purchase 40005-T |
| 25 nmol | 40005 | Purchase 40005 | |||
| CF®594 dUTP | CF®594 | 593/614 nm | 5 nmol | 40006-T | Purchase 40006-T |
| 25 nmol | 40006 | Purchase 40006 | |||
| CF®640R dUTP | CF®640R | 642/662 nm | 5 nmol | 40007-T | Purchase 40007-T |
| 25 nmol | 40007 | Purchase 40007 | |||
| CF®680R dUTP2 | CF®680R | 680/701 nm | 5 nmol | 40003-T | Purchase 40003-T |
| 25 nmol | 40003 | Purchase 40003 |
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文献和实验保持优异的水溶性，为该波段最亮的橙色荧光染料。例如：同等的标记程度下，CF543 标记的羊抗鼠IgG抗体的亮度高于用Alexa Fluor 546 标记的2~10倍。CF 555、AlexaFluor 555、Tetramethylrhodamine(TAMRA) 等匹配Cy3滤光片的橙色荧光染料；红色（568-594nm处激发）CF 568、Alexa Fluor® 568,ATTO 565, Rhodamine Red等568nm处红色荧光染料；CF 568染料耐光性最好；高效水溶
，耐光，水溶性好，确保了CF 543染料与抗体的耦联物保持优异的水溶性，为该波段最亮的橙色荧光染料。例如：同等的标记程度下，CF 543标记的羊抗鼠IgG抗体的亮度高于用Alexa Fluor 546 标记的2~10倍。 CF 555、Alexa Fluor 555、Tetramethylrhodamine (TAMRA) 等匹配Cy3滤光片的橙色荧光染料。 4、红色（568-594nm处激发） CF 568、Alexa Fluor® 568, ATTO 565
，与活化的HRP反应生成5’-HRP寡核苷酸。后者是在全成寡核苷酸过程中将一个5’带连接臂及CF3基团的尿苷3’亚磷酰亚胺合成在寡核苷酸链中，合成后此活化的寡核苷酸与AP反应即得到AP标记的寡核苷酸。 6．DNA半抗原标记 其原理与Bio-11-dTUP相同，只是用毛地黄甙代替生物素形成Dig –11- dUTP，酶促掺入DNA分子。用抗毛地黄甙抗体检测标记在DNA上的半抗原分子Digoxigenin（地高辛）
