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- Clontech
- USA
- 639622
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
1年
- 英文名：
In-Fusion Advantage PCR Cloning Kit w/NucleoSpin
- 供应商：
北京智杰方远
- 保存条件：
-20度
- 规格：
10 rxns
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文献和实验Mutagenesis by PCR(adapted from Ito et al , Gene 102, 67-70) This method needs only a single mutagenic primer (+ 3 other primers which are the same for all constructs if used in the same vector) and would be a cheap and reliable method if lots
Inverse PCR & Cycle Sequencing
2 units Taq 0.4ul Primers for PCR : type of P element 5' or 3' end forward primer reverse primer annealing temperature PZ P
Cleavage Efficiency Close to the Termini of PCR Fragments
When designing PCR experiments in which the synthesized DNA fragment is to be subsequently digested with a RE, it is very important to determine how many extra nucleotides should be added to the 5’-end of the PCR primer
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