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- 详细信息
- 文献和实验
- 技术资料
- 保质期:
1年
- 英文名:
In-Fusion Advantage PCR Cloning Kit w/Cloning Enhancer
- 供应商:
北京智杰方远
- 保存条件:
-20度
- 规格:
100 rxns
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
background. 4. Incubate tissue in X-Phos/NBT Detection Buffer for 15' at room temperature. XPhos/NBT Detection Buffer (Buffer 3 as described for Genius kit by Boehringer-Mannheim) 100 mM Tris-HCl, pH 9.5 100 mM NaCl 50 mM MgCl
Introduction to the EMSA (Gel Shift) Technique
The interaction of proteins with DNA is central to the control of many cellular processes including DNA replication, recombination and repair, transcription, and viral assembly. One technique that is central to studying gene regulation
Cloning PCR products using TA vectors
components leading to PCR failure. On the other hand, several commercially available kits for cloning PCR fragments take advantage of the ability of Taq polymerase to cause 3'-modifications. Most of these use a plasmid vector with thymidine residues
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