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- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
1年
- 英文名：
ApoAlert Caspase Profil. Plate
- 供应商：
北京智杰方远
- 保存条件：
-20度
- 规格：
1 板
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文献和实验Preparation of DNA Template For Direct Sequencing of Large Insert PAC and BAC Plasmid
reaction will require 160-300ng of template. 1. Streak clone stock to single colony on LB (+antibiotic) plate. Inoculate 1ml LB (+antibiotic) culture with single colony, grow overnight at 37?C. Using 3 ul of (overnight growth) single colony
Exposing gels and plates containing radioactive samples to X-ray film
with PPO or Enlightening or to spray a 3H-containing TLC plate with spray Enhance or some such product in order to detect tritium. In both cases exposure should be with flashed film at - 70°C. Detection of 35S and 14C. 35
Differential Interference Contrast (Nomarski, DIC, Hoffman Modulation Contrast)
as before, and look for flagella. They appear dark in phase contrast. Try to measure them using the ocular micrometer scale. Observations of living bacteria Obtain a bacterial plate or broth culture. Have handy a Fisher burner, striker, squirt bottle
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