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- 详细信息
- 文献和实验
- 技术资料
- 保质期:
1年
- 英文名:
10X Advantage 2 SA PCR Buffer
- 供应商:
北京智杰方远
- 保存条件:
-20度
- 规格:
600 μl × 2
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文献和实验Inverse PCR & Cycle Sequencing
(Sau3A I, HinP1 I, or Msp I--done separately) Set up 2 separate digestions: Genomic DNA (~2 flies) 10.0ul 10X buffer (NEB Sau3A or NEB 2) 2.5ul 100 ug/ml RNase
mineral oil (for thermocyclers without a heated lid 1. Combine the following for each reaction (on ice) in a 0.2 or 0.5 ml tube: 10X PCR buffer
well in the PCR buffer. However, you must gel-purify the digested DNA. 4) For the second PCR step, you can start the PCR without the oligo I and II, then add the oligos after a few cycles. 5) Do not use Vent polymerase for mutagenesis work.
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