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- Clontech
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- 639542
- 2025年08月21日
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
1年
- 英文名：
RNA to cDNA EcoDry™ Premix (Oligo dT)
- 供应商：
北京智杰方远
- 保存条件：
-20度
- 规格：
96 rxns
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文献和实验Isolation of RNA from Difficult Tissues
Isolation of RNA from Difficult Tissues The often exacting process of isolating intact total RNA from tissue becomes even more difficult when processing certain problematic tissues. Fibrous tissues and tissues rich in protein, DNA
and redissolve in 20 µl H 2ODEPC measure RNA concentration and check integrity on a denaturing gel II. cDNA synthesis for each RNA set up four reactions (one tube for each degenerate anchored oligo(dT) primer set - T 12MA,T12 MC
Synthesis of Radiolabeled, Subtracted cDNA Probes Using Oligo(dT) as a Primer
to the prehybridization and hybridization reactions at 1 μg/ml to prevent nonspecific hybridization between the oligo(dT) tails of the cDNA and oligo(dA) tracts in the genomic DNA.
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