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- 详细信息
- 文献和实验
- 技术资料
- 保质期:
1年
- 英文名:
Advantage cDNA Polymerase Mix
- 供应商:
北京智杰方远
- 保存条件:
-20度
- 规格:
100 次
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文献和实验, 1.6 µl 4dNTP mix (25 µM), 2 µl T12 MN primer, 2 µl cDNA, 0.2 µl Taq DNA polymerase (5 U/µl), 1 µl [a- 33P]dCTP aliquot and add 2 µl arbitrary primer (2 pmol/µl) run PCR: 40 cycles (94 °C, 30 sec.) (40 °C, 2 min.) (72 °C, 30 sec.), 1 cycle
Creating a deletion by PCR splicing
of primers combined of flanking sequences and a high fidelity polymerase (a mix of Taq and Pfu for example). The procedure is shown on the picture. The only notes which I want to make are: If you use the plasmid as a template use about 500 ng
The ribonuclease protection assay (RPA)
P]UTP 1 µl T7 RNA polymerase (Keep at -20°C until use, return to -20°C immediately). Mix by gentle pipetting or flicking and quick spin in a microfuge. Incubate at 37°C for 1 hour. 2. Terminate the reaction by adding 2 µl of DNase. Mix
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