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- 2025年08月19日
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
1年
- 英文名：
Advantage 2 Polymerase Mix
- 供应商：
北京智杰方远
- 保存条件：
-20度
- 规格：
500 次
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文献和实验Creating a deletion by PCR splicing
of primers combined of flanking sequences and a high fidelity polymerase (a mix of Taq and Pfu for example). The procedure is shown on the picture. The only notes which I want to make are: If you use the plasmid as a template use about 500 ng
The differential display protocol described here is based on the principle described by: Liang, P. and A.B. Pardee : Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction, Science 257 , 967-971
Single tube confirmation PCR protocol
Polymerase. - Add 35 µl of the PCR mix to each of the 20 PCR tubes that already contain the 15 µl of primers and template. 110 µl 5 µl 10 x Taq buffer(see below) 11 µl 0.5 µl 20 mM dNTP's (0.2 mM) 11 µl 0.5 µ
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