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- Clontech
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- 636974
- 2025年08月16日
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
1年
- 英文名：
DNA Amplification Clean-Up Kit
- 供应商：
北京智杰方远
- 保存条件：
-20度
- 规格：
1 × 96 preps
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文献和实验Materials: sterile water 10X amplification buffer with 15mM MgCl2 10 mM dNTP 50 μM
resuspend silica pellet in desired volume of 10 mM Tris 7.5 or TE or 1X restriction buffer for downstream digests heat 5 minutes at 60 degrees spin 2 min @ full speed in microcentrifuge remove DNA -containing supernatant to clean tube Notes & Misc
DNA extraction from Mutation Detection Enhancement (MDE) Gel Stained with Silver Nitrate
is normally verified using sequencing. The most important part of sequencing reaction is to prepare sufficient and clean DNA template. To enrich mutant DNA prior to sequencing, aberrantly migrated bands were excised from the SSCP gel and then re-amplified
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