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- 2025年08月17日
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
1年
- 英文名：
Terra™ qPCR Direct SYBR® Premix
- 供应商：
北京智杰方远
- 保存条件：
-20度
- 规格：
200 rxns
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文献和实验DNA extraction from Mutation Detection Enhancement (MDE) Gel Stained with Silver Nitrate
by PCR, which can be subjected to direct DNA cycle sequencing. Many different procedures are now available to recover the DNA fragments from polyacrylamide gel (4, 6). However, those techniques can take a long time and recoveries can be low for MDE.
ORNL MICROARRAY HYBRIDIZATION PROTOCOLS
Direct labeling of total RNA with Cy3 and Cy5: A. MATERIALS RNeasy® Mini Kit (Qiagen; Cat # 74106) SuperScript II RT (200U/µL) (Life Technologies; Cat # 18064-014) QIAquick PCR Purification Kit (Qiagen; Cat # 28106)
of tissue/ cell harvest. Dynabeads® mRNA DIRECT™ Kit protocols can be scaled up or down to suit specific sample source and quantity. Please see section "Sample Guidelines and Scaling" before preparing the sample, and for recommended bead and buffer volumes
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