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- Clontech
- USA
- 636055
- 2025年08月13日
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
1年
- 英文名：
CHROMA SPIN+STE-10 Columns
- 供应商：
北京智杰方远
- 保存条件：
-20度
- 规格：
20 本
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文献和实验Inverse PCR & Cycle Sequencing
A with disposable tissue grinder (Kontes). 3) Add an additional 200 µl Buffer A and continue grinding until only cuticles remain. 4) Incubate at 65° for 30 minutes. 5) Add 800 µl LiCl/KAc Solution and incubate on ice for at least 10 minutes. 6) Spin for 15
Preparation of DNA Template For Direct Sequencing of Large Insert PAC and BAC Plasmid
. If duplicates are used, transfer culture fluid from the duplicate block to the first block. Spin again, decant and drain. 3. Resuspend each pellet in 300ul of ice cold Qiagen buffer R1 with RNAse A (0.2 mg/ml) and RNAse T1 (100 units/ml). Let rest for 10
Preparation of Genomic DNA from Bacteria- using Phase Lock GelTM
. Transfer 1.5 ml to a micro centrifuge tube and spin 2 min. Decant the supernatant. Repeat with another 1.5 ml of cells. Drain well onto a Kimwipe. 2. Resuspend the pellet in 467 μl TE buffer by repeated pipetting. Add 30 μl of 10% SDS and 3 μl
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