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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保质期:
1年
- 英文名:
Stellar™ Competent Cells
- 供应商:
北京智杰方远
- 保存条件:
-20度
- 规格:
10 transformations
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文献和实验Transformation of Competent Cells
Transformation of Competent Cells A. Preparing competent cells. Inoculate 30 ml of SOB broth in a 250-ml flask with bacteria to be transformed from a single colony on a fresh plate.
How to Make Competent CellsStep 1: Take an 100 m L aliquot of frozen cells from the -80o C and innoculate about 500 ml to 1 L sterile LB broth . DO NOT ADD antibiotic, since these cells do not have an plasmid in them. Work as sterile as possible
Procedure for Transfection of Mammalian Cells
Materials: Lipofectamine IMDM containing 10% fetal bovine serum, 1% glutamine, 1% aa IMDM containing 1% glutamine
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