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- 详细信息
- 文献和实验
- 技术资料
- 保质期:
1年
- 英文名:
Human Salivary Gland Total RNA
- 供应商:
北京智杰方远
- 保存条件:
-20度
- 规格:
50 μg
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文献和实验Isolation of RNA from Difficult Tissues
Isolation of RNA from Difficult Tissues The often exacting process of isolating intact total RNA from tissue becomes even more difficult when processing certain problematic tissues. Fibrous tissues and tissues rich in protein, DNA
When isolating RNA, it helps to have a rough idea of how much total or poly(A) RNA can be recovered from a given amount of tissue or cells. It is also beneficial to know rough expression levels for rare to moderately abundant transcripts
(1.6ml/220 ml), stable for 1 month at RT 2 M NaOAc pH 4.1 (100 ml): dissolve 27.2 g NaOAc in about 54 ml H2 O and add about 46 ml conc HAc Remarks: As usual when working with RNA: pay
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