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- 详细信息
- 文献和实验
- 技术资料
- 保质期:
1年
- 英文名:
Mate & Plate™ Library - Universal Human (Normalized)
- 供应商:
北京智杰方远
- 保存条件:
-20度
- 规格:
5 × 1 ml
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文献和实验Two-hybrid analysis of genetic
mate to form diploids,the YPD plate is pressed to a new replica velvet and the impression is lifted with a plate containing diploid selection medium and an indicator like X-Gal.Blue patches (dark spots)on the X-Gal plate indicate that the lacZ reporter
Introduction pBeloBAC11 vector allows lacZ-based positive color selection of the BAC clones that have insert DNA in the cloning sites at the time of library construction. Because the vector exist
Clone Genes From a Phage Library
vector (at Eco RI). Its complexity is roughly 80,000 X an insert size of 4 to 8 kb = 320 Mbp, which is about 20-fold coverage of this yeast genome. The aim is to evenly plate out about 10,000 phage over about 10 plates. What you need in addition
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