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- 详细信息
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- 技术资料
- 保质期：
1年以上
- 英文名：
DIG High Prime DNA Labeling and Detection Starter Kit II：Kit for chemiluminescent detection with CSPD, ready-to-use
- 库存：
现货
- 供应商：
北京智杰方远
- 保存条件：
-20度冻存
- 规格：
12LR;24DR
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DIG高效DNA标记及检测Starter Kit II说明书
| DIG高效DNA标记及检测Starter Kit II | |||
| DIG High Prime DNA Labeling and Detection Starter Kit II：Kit for chemiluminescent detection with CSPD, ready-to-use | |||
|
对于需要使用化学发光法检测的用户，我公司现提供了此新的DIG标记及检测试剂盒。
与上述的StarterKit I不同，此新的StarterKit II所含的底物为CSPD，但它同样有以下特点：
●能对10×10cm印迹膜作12个标记反应及24个检测反应，所以特别适合初用者及需处理较少量样品的用户使用。 ●包含DIG高效标记试剂，令标记效率更高，操作更方便。 ●包括DIG定量/对照试纸条及NBT/BCIP浓缩液，令您更易估计探针浓度，从而取得理想的信噪比。 |
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| 产品号 | 产品规格 | 厂商 | |
|
1585614
11585614910
|
Kit：1 kit (12 labeling reactions and 24 detection reactions) | Roche | |
DIG-High Prime DNA Labeling and Detection Starter Kit II
Application
DIG-High Prime labeled probes can be used in northern, Southern-, and dot-blot analysis, as well as colony and plaque hybridizations. This kit offers random-primed labeling of DNA templates with DIG-11-dUTP, alkali-labile, and chemiluminescent detection of the DIG-labeled hybrids. This kit was assembled with convenience in mind, offering ready-to-use CSPD supplied with a dripping device for easy application, ready-made blocking solution, and DIG Easy Hyb granules. The DIG-High Prime mixture includes stabilized Klenow enzyme, nucleotides, primers, and reaction buffer, all in one convenient reagent.
Benefits
The High Prime method of labeling offers many benefits: in addition to the optimized labeling efficiency, plasmid, cosmid, and phage DNA, linearized or supercoiled can be used as templates, yielding the same labeling efficiency.
Product Description
Sensitivity and specificity: Standard assay uses 1 µg of linearized pBR328. It is usual to expect 0.8 µg labeled DNA after 1 h of labeling, and 2.3 µg labeled DNA after 20 h reaction. In a dot blot hybridization, 0.03 pg of homologous DNA is detectable with chemiluminescence (probe concentrations at 20 ng/ml). A single copy human gene (t-PA) is detectable in a Southern blot, loading 1 µg of digested placenta DNA.
Contents
1. DIG-High Prime, 5x conc., 50 µl
2. DIG-labeled Control DNA, 20 µl, (5 µg/ml) pBR328 (linearized with Bam HI)
3. DNA Dilution Buffer, 3 x 1 ml
4. Anti-digoxigenin-AP Conjugate, 50 µl
5. CSPD, ready-to-use, 50 ml
6. Blocking Solution, 10x conc., 4 x 100 ml
7. DIG Easy Hyb Granules, 4 x 100 ml
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