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文献和实验SDS-PAGE Western Blotting Protocols
-PAGE, although it does reduce the effective size of the resolving gel. 8. Add upper layer of water-saturated n-butanol to the top of the gel. 9. Let polymerize (this takes ~10-15’). 10. Invert gel to remove n-butanol. Touch with filter paper to wick off residual
Western Blotting and Immunostaining
away trapped air between the gel and membrane. If necessary, reposition the membrane once. Working quickly, place a soaked piece of blotting paper on top of the membrane, followed by 3 to 4 soaked blotting pads. The last pad should be about 1 cm
做了两个月的时间的 western blotting 终于出了比较好的结果。 老板让整理出protocol 这是写的PROTOCOL 大概会有很多错误请大家指正!谢谢先 WESTERN PROTOCOL A. Preparation of Samples 1. Get the heads of the fly 2. Stir them in the lysis buffer(4heads/10ul) 3. Add 2XSDS-gel-loading buffer
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