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- 详细信息
- 文献和实验
- 技术资料
- 应用范围:
Western Blotting
- 宿主:
0
- 库存:
大量
- 抗原来源:
0
- 是否单克隆:
0
- 规格:
100
- Fluorescent
- Chemifluorescence
- Chromogenic
- Chemiluminescence
Product Family Information
Overview of the SNAP i.d.® Protein Detection SystemSuperior Blots in a SNAP! |
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Immobilon Transfer Membranes, Sandwiches, and Blotting Filter PaperMillipore offers three different Immobilon PVDF membranes, each optimized for different protein blotting applications. We also now offer blotting sandwiches that feature pre-cut sheets of membrane and blotting filter paper. |
更多产品技术资讯,请访问密理博中国博客:http://blog.milliporechina.com
详细描述见链接:http://www.millipore.com/catalogue/item/IBFP0785C
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文献和实验SDS-PAGE Western Blotting Protocols
-PAGE, although it does reduce the effective size of the resolving gel. 8. Add upper layer of water-saturated n-butanol to the top of the gel. 9. Let polymerize (this takes ~10-15’). 10. Invert gel to remove n-butanol. Touch with filter paper to wick off residual
Western Blotting back to top Detection Substrates Chromogenic Detection Prepare the substrate according to manufacturer’s instructions. Place the blot in a clean container and add substrate to completely
免疫印迹(Western Blotting)的基本原理及应用 免疫印迹是一个用于蛋白质分析的常规技术,在电场的作用下将电泳分离的蛋白从凝胶转移至一种固相支持物,然后利用抗原-抗体的特异性反应,从蛋白混合物中检测出目标蛋白,从而定量或定性的确定正常或实验条件下细胞或组织中目标蛋白的表达情况。Western blotting还可用于蛋白-蛋白、蛋白-DNA和蛋白-RNA相互作用的后续分析,作为一种廉价、便捷、可靠的研究工具,将与质谱和蛋白质芯片等技术一起在蛋白质组时代发挥重要作用
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