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- 文献和实验
- 技术资料
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不限
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大量
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不限
- 供应商:
西宝生物
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0
- 保存条件:
Store at RT,
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50 sheets
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文献和实验A protocol for cleaning and reusing the large 25 x 25 cm plates
We regularly reuse our large 25x25 cm plating trays; initially, however, we were plagued by gross microbiological contamination when reusing the trays. Various combinations of cleaning with a laboratory dishwasher, ethyl alcohol, and UV
SDS-PAGE Western Blotting Protocols
glass plate can be treated with Rain-X to facilitate later removal of the gel. 2. Slide into holder without tightening the screws. 3. Place on pouring stand, making sure that the glass plates are pushed all the way to the bottom before tightening
。短暂低速离心后,立即放置于冰上5 min。 5.5 电泳: 5.5.1 将RNA样品小心加到点样孔中。 5.5.2 在5 V/cm下跑胶(5×14cm)。在电泳过程中,每隔30 min短暂停止电泳,取出胶,混匀两极的电泳液后继续电泳。当胶中的溴纷蓝(500bp)接近胶的边缘时终止电泳。 5.5.3 紫外灯下,检验电泳情况,并用尺子测量18S、28S、溴纷蓝到点样孔的距离。 注意不要让胶在紫外灯下曝光太长时间。 5.6. 转膜
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