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- BioVision
- 2805-10
- 2025年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
EZSolution Blasticidin S Hydrochloride, Sterile-Filtered ;Blasticidine S
- 克隆性：
多克隆
- 保存条件：
见包装
- 规格：
10ml
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文献和实验Guanidine Hydrochloride Purification of Proteins From SDS
Pepstatin A (2.0 mg/ml)10 ml Leupeptin (1.0 mg/ml)10 ml Aprotinin (5.0 mg/ml)(Protease Inhibitor Cocktail Sigma #P8340)Denaturation Buffer6 M guanidine hydrochloride 57.3 g G-HCl20 mM Hepes 7.9 2 ml 1M Hepes pH 7.910 mM KCl 0.074 g KCl0.5 mM DTT 100 ml 0.5 M
Guanidine Hydrochloride Purification of Proteins From SDS PAGE
(Sigma #P7626) 10 ml Pepstatin A (2.0 mg/ml) 10 ml Leupeptin (1.0 mg/ml) 10 ml Aprotinin (5.0 mg/ml) (Protease Inhibitor Cocktail Sigma #P8340) Denaturation Buffer 6 M guanidine hydrochloride 57.3 g G-HCl 20 mM Hepes 7.9 2 ml 1M Hepes pH 7.9 10 mM
Protocols for the preparation of tumour cells for s.c. injections in mice
, 1% antibiotic solution (penicillin, streptomycin, fungizone) and filtered glucose (0.37%). Flasks: Small-size flasks (25 cm2 ) are filled with 8-10 ml of medium. Medium-size flasks (75 cm2 ) are filled with 20 ml of medium. Large-size flasks (150 cm
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