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- 英文名：
RNase-Free DNase Set (50)
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文献和实验Ribonuclease A (RNase A), DNase-free
FeatureThe RNase A is free of DNase activity. It is not necessary to heat it before use.DescriptionThe Ribonuclease A (RNase A) is an endoribonuclease that specifically degrades single-stranded RNA at C and U residues. It cleaves the phosphodiester
Preparation of RNase-Free DNase by Alkylation
with electrophoresis. DNase treatment makes the mixture more soluble, even if DNA degradation is only partial.
1-2 ug of RNA on a 1.3 % agarose/formaldehyde gel to check integrity of RNA.Reagents/SolutionsRneasy Mini Kit （Qiagen）RNase-Free DNase Set （50） （Qiagen）100 % Ethanol80% ethanolTipsExpect to lose about 1/3 of the RNA by irreversible binding
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