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DirectPrep 96 Miniprep Kit (4)
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文献和实验freeshotgun sequencing library from miniprep BAC DNA
Miniprep of BAC DNA 1.Inoculate 4 - 8 tubes of 3ml/tube LB with 15μg/ml chloramphenicol with BAC clone and grow oveernight. Miniprep with AutoGen 740 according to the protocol described in our Web page. DNA pellet
free shotgun sequencing library from miniprep BAC DNA
Miniprep of BAC DNA 1.Inoculate 4 - 8 tubes of 3ml/tube LB with 15μg/ml chloramphenicol with BAC clone and grow oveernight. Miniprep with AutoGen 740 according to the protocol described in our Web page. DNA pellet were resuspended in 265 μl TE
Construction of Gene-Targeting Vectors by Recombineering
. coli cells is not as stringent as other commonly used antibiotics. Therefore, we do not recommend the use of Puro selection in 96-well liquid media culture. As an alternative to using the Fast-Media Kit, one can prepare media using 50-100 µg
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