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MinElute Reaction Cleanup Kit (50)
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文献和实验Sequencing Reaction Cleanup in 384 Well Sequencing Reaction Plates
the ABI 3700, the dryed reaction products should be dissolved in 20 μl of water and then loaded onto the ABI 3700 as recommended. Sit back and enjoy 5 to 10 times higher signal strength than with the Sephadex cleanup. ** Note: It is highly likely
Materials For purifying plasmid DNA from Escherichia coli cells, the Qiagen Spin Miniprep Kit produces quite reliable results. Do not autoclave solutions containing isopropanol or MOPS; use sterile filtration if necessary.
Clean-up Spin Protocol For DNA fragments (30bp-100 bp) 1) Add 3 x volume of buffer P3, 3 x volume of isopropanol to the enzymatic reaction and mix throughly with pipetting or vortex. The maximum volume of the reaction can be processed
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