AssayPro—Human IgD ELISA Kit（免

Introduction
Immunoglobulin D (IgD) is an antibody of the immunoglobulin isotype that is a monomer made up two identical heavy and light chains organized into variable and constant domains similar to IgG. It has soluble and transmembrane forms and is found as an antigen receptor on the surface of B cells (1). IgD is an important immunomodulatory molecule that promotes immune defense. Overactivation of this pathway can cause inflammation and tissue damage (2). Serum IgD was increased in patients with AIDS, tuberculosis, infectious hepatitis, and respiratory diseases (3-4).

Principle of the Assay
The AssayMax Human IgD ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of human IgD in plasma, serum, urine, saliva, milk, and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures human IgD in less than 4 hours. A polyclonal antibody specific for human IgD has been pre-coated onto a 96-well microplate with removable strips. IgD in standards and samples is sandwiched by the immobilized antibody and the biotinylated polyclonal antibody specific for IgD, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.