AssayPro—Human Hemopexin ELISA

Introduction
Hemopexin is a heme-binding plasma glycoprotein which, after haptoglobin, forms the second line of defense against hemoglobin-mediated oxidative damage during intravascular hemolysis. A decrease in plasma hemopexin concentration reflects a recent release of heme compounds in the extracellular compartment. Heme–hemopexin complexes are delivered to hepatocytes by receptor-mediated endocytosis after which hemopexin is recycled to the circulation (1). Studies indicated that increased hemopexin level associate with minimal change disease (2), sporadic Alzheimer's disease (3), heavy-drinking chronic alcoholics (4), hemolytic anemias, chronic neuromuscular diseases and acute intermittent porphyria (5).

Principal of the Assay
The AssayMax Human Hemopexin ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of human hemopexin in plasma and serum. This assay employs a quantitative competitive enzyme immunoassay technique that measures human hemopexin in less than 2 hours. A polyclonal antibody specific for human hemopexin has been pre-coated onto a 96-well microplate with removable strips. Hemopexin in standards and samples is competed by a biotinylated hemopexin sandwiched by the immobilized antibody and streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.