Human Ceruloplasmin ELISA Kit（

Introduction
Ceruloplasmin is an abundant α2-serum glycoprotein that contains 95% of the copper found in the plasma of vertebrate species (1). Ceruloplasmin is a copper-binding protein that normally removes iron from cells by its ferroxidase activity. Ceruloplasmin concentration on average is 14.6 (±4.0) mg/dl (2). Low levels of ceruloplasmin lead to the abnormal deposition of iron in cells, including those of the pancreas, liver, retina and the basal ganglia region of the brain. Some diseases associated with ceruloplasmin are Wilson's disease (3), Hemochromatosis (4), Menkes disease, (5) and Aceroluplasminemia (1).

Principle of the Assay
The AssayMax Human Ceruloplasmin ELISA kit is designed for detection of ceruloplasmin in human urine, saliva, milk, and cell culture supernatant. This assay employs a quantitative sandwich enzyme immunoassay technique that measures ceruloplasmin in less than 4 hours. A polyclonal antibody specific for ceruloplasmin has been pre-coated onto a microplate. Ceruloplasmin in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for ceruloplasmin, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.