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- Life Technologies
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- 2025年07月14日
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文献和实验Blotting: 1. Remove gel from plate-sandwich (note L to R orientation), cut away stacking gel and agar plug (if use), rinse running gel briefly in semi-dryTransfer Buffer (sdTB): 2. Cut six Whatman filter papers and one Immobilon-P filter to the size
in methanol at room temp. 3. Remove methanol and add 1x Blotting buffer until ready to use. G. Membrane transfer 1. Assemble "sandwich" for Bio-Rad's Transblot. 2. Prewet the sponges, filter papers (slightly bigger than gel) in 1x Blotting buffer
constant voltage for 3 hours at 15o C. Assemble the blotting sandwich as described in chapter 5 of this book. or a semi-dry apparatus with a solution containing Tris (13 mM), glycine (100 mM) and methanol (20% v/v anodic side; 5% v/v cathodic
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