Magnetic Co-Immunoprecipitatio

产品名称	Magnetic Co-Immunoprecipitation (Co-IP)Kit
产品货号	Bes3011(S)
规格	12T
官网品名	Co-IP试剂盒
官网产品页

官网产品详情

实验原理

细胞在非变性条件下被裂解时，细胞内许多 “蛋白质 - 蛋白质”相互作用复合体被完整保留下来。利用目标蛋白质的特异性抗体进行免疫沉淀，再通过变性凝胶电泳分离所得蛋白质复合体，以此定性或定量分析几种蛋白质之间的相互作用力。

产品优势

1. 伯信独立研发，具有自主知识产权。

2. 配备完整：该试剂盒包含交联剂，可使抗体与琼脂糖珠交联，洗脱时只收集诱饵蛋白和捕获蛋白。

3. 同一个抗体既可用于免疫沉淀，又可用于 WB 检测。

4. 操作简单、耗时短、结果客观准确。

技术流程

详见产品说明书 ： Bes3011 Co-IP

文章案例

A Article： Super enhancer-driven LINC01013 mediates hypoxia-induced mitochondrial dysfunction by HSPA9 to determine pulmonary arterial smooth muscle cell fate Periodicals：Cellular and Molecular Life Sciences IF：6.2001

Article ： Elevated Dickkopf 3 Promotes Abdominal Aortic Aneurysm Formation via Modulated Phenotype Switch of Vascular Smooth Muscle Cells.

Periodicals ：Research（Washington,D.C.）

IF ： 10.700

Article ： Reduced Wiskott-Aldrich syndrome protein expression in preeclampsia placenta impairs trophoblast syncytialization by modulating syncytin-2 via FAK/β-catenin pathway.

Periodicals ：Frontiers in Cell and Developmental Biology

IF ：4.300

Article ： SIRT7 ameliorates Th17/Treg imbalance by desuccinylation of STAT3 to improve immune thrombocytopenia.

Periodicals ：Clinical & Translational Immunology

IF ：3.800

Article ： m5C-modified circRREB1 promotes lung cancer progression by inducing mitophagy.

Periodicals ：Journal of Experimental & Clinincal Cancer Research: CR

IF ：12.800

Article ： Lactate accumulation induces H4K12la to activate super-enhancer-driven RAD23A expression and promote niraparib resistance in ovarian cancer.

Periodicals ：Molecular Cancer

IF ：27.700

Article ： o8G-modified circPLCE1 inhibits lung cancer progression via chaperone-mediated autophagy.

Periodicals ：Molecular Cancer

IF ：27.700

Article ： CircAKT3 promotes prostate cancer proliferation and metastasis by enhancing the binding of RPS27A and RPL11.

Periodicals ：Molecular Cancer

IF ：27.7 00

Article ： LncRNA-MEG3 Regulates Muscle Mass and Metabolic Homeostasis by Facilitating SUZ12 Liquid-Liquid Phase Separation.

Periodicals ：Advance Science (Weinheim, Baden-Wurttemberg, Germany)

IF ：14.100

Article ： GILT stabilizes cofilin to promote the metastasis of prostate cancer.

Periodicals ：Cell death discovery

IF ：7.0 00

Article ： Exosome-derived hsa_circ_0007132 promotes lenvatinib resistance by inhibiting the ubiquitin-mediated degradation of NONO.

Periodicals ：Non-coding RNA Research

IF ：4.7 00

Article ： Exosomal AFAP1-AS1 Promotes the Growth, Metastasis, and Glycolysis of Pituitary Adenoma by Inhibiting HuR Degradation.

Periodicals ：Molecular Neurobiology

IF ：4.6 00

Article ： Circular RNA circ_0004470 accelerates the occurrence of lung cancer by promoting DNA damage and cell cycle arrest.

Periodicals ：The Journal of Biological Chemistry

IF ：4.0 00

Article ： NSUN5 promotes cholangiocarcinoma progression by enhancing GLS mRNA stabilization.

Periodicals ：Journal of Cancer Research and Clinical Oncology

IF ：2.7 00

Article ： Hepatocyte-derived Fetuin-A promotes alcohol-associated liver disease in mice by inhibiting autophagy-lysosome degradation of TLR and M2 macrophage polarization.

Periodicals ：Free Radical Biology & Medicine

IF ：7 .100

Article ： The long noncoding RNA ELFN1-AS1 promotes gastric cancer growth and metastasis by interacting with TAOK1 to inhibit the Hippo signaling pathway.

Periodicals ：Cell death discovery

IF ：6.100

Article ： circSORBS1 inhibits lung cancer progression by sponging miR-6779-5p and directly binding RUFY3 mRNA.

Periodicals ： Journal of Translational Medicine

IF ： 6.100

Article ： HNRNPA2B1 stabilizes NFATC3 levels to potentiate its combined actions with FOSL1 to mediate vasculogenic mimicry in GBM cells.

Periodicals ：Cell biology and Toxicology

IF ：5.300

Article ： TRMT10C-mediated m7G modification of circFAM126A inhibits lung cancer growth by regulating cellular glycolysis.

Periodicals ：Cell Biology and Toxicology

IF ：5.300

Article ： Ubiquitination of angiotensin-converting enzyme 2 contributes to the development of pulmonary arterial hypertension mediated by neural precursor cell-expressed developmentally down-regulated gene 4-Like.

Periodicals ：Respiratory Research

IF ：4.700

Article ： GBA3 promotes fatty acid oxidation and alleviates non-alcoholic fatty liver by increasing CPT2 transcription.

Periodicals ： Aging

IF ：3.900

Article ： CircFOXP1 alleviates brain injury after acute ischemic stroke by regulating STAT3/apoptotic signaling

Periodicals ： Translational Research

IF ： 10.170

Article

The short inverted repeats-induced circEXOC6B inhibits prostate cancer metastasis by enhancing the binding of RBMS1 and HuR

Periodicals ： Molecular Therapy

IF ： 12.400

Article ： 5mC and H3K9me3 of TRAF3IP2 promoter region accelerates the progression of translocation renal cell carcinoma

Periodicals ： Biomarker Research

IF ：11.100

Article ： PAK6 promotes homologous-recombination to enhance chemoresistance to oxaliplatin through ATR/CHK1 signaling in gastric cancer

Periodicals ： Cell Death and Disease

IF ： 9.000

Article ： Coordinated transcriptional and post-transcriptional epigenetic regulation during skeletal muscle development and growth in pigs

Periodicals ： Journal of Animal Science and Biotechnology

IF ：7.000

Article ： Transcription factor ZNF22 regulates blood-tumor barrier permeability by interacting with HDAC3 protein

Periodicals ： Frontiers in Molecular Neuroscience

IF ：4.800

Article ： Bmal1 Regulates Macrophage Polarize Through Glycolytic Pathway in Alcoholic Liver Disease

Periodicals ： Frontiers in Pharmacology

IF ：5.600

Article ： MicroRNA393 is involved in nitrogen-promoted rice tillering through regulation of auxin signal transduction in axillary buds

Periodicals ： Scientific Reports

IF ： 4.600