Human Alveolar Macrophages

Flow Cytometric Analysis of Blood Monocytes and Alveolar Macrophages

, and migration to sites of tissue injury and inflammation (1 ). There is also mounting evidence that recruited blood monocytes and resident alveolar macrophages (AM) in lung disease express an activated phenotype, suggesting that they may play important roles

Isolation and Culture of Human Macrophages

Methods to isolate and culture human monocyte-derived macrophages and alveolar macrophages are described. Monocytes are obtained from buffy-coat preparations by Ficoll density gradient centrifugation, followed by adhesion-mediated

Human Regulatory Macrophages

of human M regs from peripheral blood monocytes and methods for the assessment of their phenotype. The uniqueness of the human M reg is best appreciated when the M reg is compared to macrophages in other states of activation; therefore, protocols