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现货
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- 供应商:
上海然其生物科技有限公司
- 规格:
50支/包/10包/箱
| 规格: | 50支/包 | 产品价格: | ¥188.4 |
|---|---|---|---|
| 规格: | 10包/箱 | 产品价格: | ¥1884.0 |
NUNC(Thermo)339650 15 mL 锥形离心管 中英文产品介绍
中文产品介绍
产品简介
Nunc 15 mL 无菌锥形离心管(货号339650),管身聚丙烯(PP),蓝色 HDPE 螺帽,Bulk 散装无支架包装;γ 射线辐照灭菌(SAL 10⁻⁶),无 RNase/DNase、无热原、无细胞毒性,满足 USP Class VI 生物相容性标准Thermo Fis...。最大相对离心力10500 × g(转子完全支撑条件);管身印刷清晰刻度,超大标记书写区;密封螺帽抗漏液。适用于细胞沉淀、核酸蛋白样本处理、试剂配制分装、生物样本暂存,广泛用于细胞、分子生物学实验Capitol Sc...。
- 货号:339650
- 容积:15 mL,锥形底
- 材质:管身聚丙烯 PP;管盖 HDPE(蓝色)
- 包装规格:50 支 / 小包,500 支 / 整箱(Bulk 散装,不含支架)documents....
- 最大 RCF:10500 × g(转子完整支撑)
- 灭菌:γ 辐照无菌 SAL 10⁻⁶
- 质控:无 DNase/RNase、无热原、无细胞毒性,TSE/BSE‑free,USP Class VI
主要特点
- 高强度聚丙烯材质,耐受较高转速离心;边缘密封螺帽,颠倒混匀、离心过程不易漏液,保护珍贵样本。
- 印刷清晰体积刻度,超大书写标记区域,方便样本编号与溯源。
- 高洁净等级,无核酸酶、低内毒素,适配细胞培养、核酸提取、蛋白纯化等敏感实验。
- Bulk 散装包装,适合大批量实验;同规格带支架版本货号339651,管体物理参数完全一致,仅包装形式不同Thermo Fis...。
- 一次性耗材,禁止高压灭菌重复使用。
下游应用
细胞离心收集沉淀;菌体沉淀;核酸、蛋白样本制备;试剂配制分装;生物样本暂存;分子与细胞生物学样本前处理。
储存条件
室温干燥避光存放;一次性使用,禁止高压灭菌。
⚠️提示:10500×g 额定值需要转子完整支撑;悬空放置离心务必降低转速。
English Product Introduction
Product Description
Nunc 15 mL sterile conical centrifuge tubes (Cat.No.339650). Tube body made of polypropylene (PP), blue HDPE screw‑cap, bulk‑packaged without rack. Gamma‑irradiated sterile (SAL 10⁻⁶), RNase‑/DNase‑free, non‑pyrogenic, non‑cytotoxic, USP Class VI certifiedThermo Fis.... Maximum RCF up to 10500 × g (full rotor support). Printed graduations and large marking‑writing area on tube wall; edge‑sealed leak‑proof screw cap. Widely used for cell pelleting, nucleic‑acid & protein sample processing, reagent preparation and temporary sample storage in molecular‑ and cell‑biology experiments.
- Catalog No.: 339650
- Volume: 15 mL, conical‑bottom
- Material: Tube: polypropylene (PP); Cap: HDPE (blue)
- Packing: 50 tubes/pouch; 500 tubes/case, bulk‑packaged (no rack)documents....
- Max RCF: 10500 × g (full conical rotor support)
- Sterilization: Gamma‑irradiation, SAL 10⁻⁶
- Quality: DNase‑/RNase‑free, non‑pyrogenic, non‑cytotoxic, TSE/BSE‑free, USP Class VI biocompatibility grade
Key Features
- High‑strength polypropylene tolerates moderate‑high‑speed centrifugation. Edge‑sealed screw cap prevents leakage during centrifugation and repeated inversion to protect valuable samples.
- Clear printed volume graduations and extra‑large writing area facilitate sample labelling and traceability.
- Ultra‑clean grade, nuclease‑free and low‑endotoxin, compatible with nuclease‑sensitive workflows including cell culture, nucleic‑acid extraction and protein purification.
- Bulk‑pack format suitable for high‑throughput experiments. Racked counterpart: Cat.No.339651Thermo Fis....
- Single‑use consumable. Do not autoclave.
Applications
Cell harvesting and pelleting; bacterial pellet collection; nucleic‑acid and protein sample preparation; reagent aliquoting; temporary biological‑sample storage; pre‑treatment workflows for molecular and cell biology.
Storage
Store at room‑temperature in dry and dark environment. Single‑use only, autoclaving prohibited.
Note: 10500 × g rating requires full rotor support; reduce RCF if tubes are not fully seated.
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文献和实验- Bio‑Protocol,荧光素酶高通量小分子筛选,试剂表直接登记货号 339650,用于细胞重悬、样本分装前处理,同时配套 339652 50 mL 管使用。
15 ml centrifuge tube (Thermo Scientific Nunc, catalog number: 339650)
supplement,青霉素、链霉素各100U/ml,EGF 20 ng/mL,b-FGF 10ng/mL。用火焰抛光的吸管反复吹打,分散组织制成单细胞悬液,吸取9滴细胞悬液、1滴0.4℅台盼蓝溶液混合,用血细胞计数板在3分钟内计数出活细胞和死细胞(台盼蓝排斥法),调整细胞密度,以105个/ mL接种75mL培养瓶中(NUNC), 于5℅CO2 培养箱, 37℃培养。期间倒置显微镜下观察细胞的生长状况。 ②传代培养:当神经球直径大约100um时收集细胞于离心管中,600转离心5分钟,弃上清,加入无血清培养基重悬
Preparation of NeoR Murine Embryonic Fibroblasts
anesthesia) or CO2 asphyxiation. Prepare three 10cm dishes containing 15ml PBS each. With the mouse on its back, wipe down the abdominal skin and fur with 70% alcohol. Make an incision down the midsection using scissors, thereby exposing the uterine horns
Preparation of NeoR Murine Embryonic Fibroblasts
to a heterozygote female. 13.5 days to 14.5 days after the plug is observed, sacrifice the pregnant female either by cervical dislocation (after appropriate anesthesia) or CO2 asphyxiation. Prepare three 10cm dishes containing 15ml PBS each. With the mouse
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