NUNC 159910 175cm细胞培养瓶,过滤盖

Nunc™ EasYFlask™ 175 cm² 细胞培养瓶（过滤盖）货号：159910

中文产品介绍

产品简介

Nunc EasYFlask 175 cm²（T175）斜颈细胞培养瓶，配备0.22 μm 疏水 PTFE 过滤透气盖；Nunclon™ Δ 等离子 TC 贴壁处理表面，高透明医用聚苯乙烯，γ 射线辐照灭菌；无 DNase/RNase、无热原、无细胞毒性，符合 USP Class‑VI 标准。滤膜在保证 CO₂/O₂气体交换的同时阻隔微生物侵入，适合大规模贴壁细胞扩增，适配 CO₂细胞培养箱。

• 货号：159910
• 培养面积：175 cm²
• 瓶盖类型：0.22 μm 过滤盖，HDPE 材质；旋转 1/3 圈完成开关，Y 形标识可视化区分透气 / 密封档位
• 推荐工作液体积：55 mL；总容积 660 mL
• 瓶颈：斜颈大口设计
• 包装规格：5 个 / 小包，30 个 / 整箱；箱内附赠备用瓶盖
• 灭菌：γ 射线辐照灭菌（SAL 10⁻⁶）
• 材质：瓶体聚苯乙烯；瓶盖 HDPE；疏水过滤膜 PTFE

主要特点

1. Nunclon™ Δ 等离子表面处理：对肿瘤细胞、原代细胞均有优异贴壁效果，批间一致性稳定，适配多种哺乳动物细胞系。
2. 0.22 μm 疏水过滤盖：无需拧开瓶盖即可完成无菌气体交换，降低污染风险；旋转瓶盖可切换密封模式用于转运样本，堆叠孵箱时 Y 标识清晰可见。
3. 人体工学斜颈大开口，移液器、细胞刮刀可以完整抵达瓶底全部生长面；瓶身两侧同时带有模刻 + 印刷双重体积刻度，方便培养基定量。瓶壁微倾斜，显微镜下可直接观察细胞汇合度。
4. 堆叠结构设计，提升孵箱空间利用率；无动物源组分；一次性使用，禁止高压灭菌。

下游应用

大规模贴壁细胞传代扩增，大量细胞收获，细胞转染，药物大批量处理实验，悬浮细胞 CO₂孵箱培养。

储存条件

室温干燥避光存放，远离高温；不可高压灭菌。

⚠️提示：孵箱内置于 Y 标识透气档位；样本转运时切换为密封档位。

English Product Introduction

Product Description

Nunc™ EasYFlask™ 175 cm² cell‑culture flask (Cat.No.159910), angled‑neck design with 0.22 μm hydrophobic PTFE filter cap. Nunclon™ Δ plasma‑treated tissue‑culture surface, high‑purity virgin polystyrene, gamma‑irradiated sterile, DNase‑/RNase‑free, non‑pyrogenic and USP Class‑VI compliant. The filter cap enables sterile gas‑exchange while preventing microbial contamination for incubation inside CO₂ incubatorFisher Sci....

• Catalog No.: 159910
• Growth area: 175 cm²
• Cap: 0.22 μm filter cap (HDPE material); one‑third‑turn opening‑closing; “Y” marker for visual vent‑on / vent‑off indication
• Recommended working volume: 55 mL; total volume 660 mL
• Neck: angled wide neck
• Pack size: 5 flasks per inner bag, total 30 per case; extra spare caps included in each case
• Sterilization: Gamma irradiation (SAL 10⁻⁶)
• Material: Flask body: polystyrene; cap: HDPE; filter membrane: hydrophobic PTFE

Key Features

1. Nunclon™ Δ surface: Reliable cell attachment for cell lines and primary cells, excellent lot‑to‑lot consistency for diverse mammalian cells.
2. 0.22 μm hydrophobic filter cap: Permits sterile gas‑exchange without cap loosening to reduce contamination risk. Rotate cap to sealed position for sample transportation. “Y” indicator remains readable even when stacked in incubator.
3. Ergonomic angled wide neck for full access by pipettes and cell scrapers. Dual molded & printed volume graduations on both sides. Slightly angled side wall allows direct microscopic observation of cell confluency.
4. Stack‑optimized footprint for efficient incubator usage. Animal‑component‑free. Single‑use only, do not autoclave.

Applications

Large‑scale adherent cell expansion, bulk cell harvesting, cell transfection, high‑volume drug‑treatment assays, suspension‑cell culture in CO₂ incubator.

Storage

Store at room‑temperature, dry and dark environment. Do not autoclave.

Note: Keep cap at vent position for incubator culture; switch to sealed mode for sample transport.