NUNC 156367 25cm细胞培养瓶,过滤盖

Nunc™ EasYFlask™ 25 cm² 细胞培养瓶（过滤盖）货号：156367Thermo Fis...

中文产品介绍

产品简介

Nunc EasYFlask 25 cm²（T25）细胞培养瓶，弯颈大口设计，配备0.2 μm 疏水性 PTFE 过滤透气盖；Nunclon™ Δ 等离子贴壁处理表面，透明聚苯乙烯材质，γ 射线辐照灭菌，无热原、无细胞毒性，无动物源组分，满足 USP Class‑VI 生物安全标准。适配 CO₂细胞培养箱，滤膜实现无菌气体交换，阻隔细菌真菌污染，用于贴壁细胞及部分悬浮细胞体外扩增培养。

• 货号：156367
• 培养面积：25 cm²
• 推荐工作液体积：7 mL
• 包装规格：10 个 / 小包，200 个 / 整箱
• 瓶盖：HDPE 高密度聚乙烯过滤盖；仅需旋转 1/3 圈即可开关；瓶盖上 “Y” 标识，堆叠存放也可直观区分透气 / 密封档位上海玮驰。

主要特点

1. Nunclon™ Δ 等离子处理表面：广谱适配肿瘤细胞系、原代细胞，提升细胞贴壁、增殖与分化性能，批间稳定性优异。
2. 0.2 μm 疏水过滤盖：自由交换 CO₂/O₂，阻挡微生物侵入；培养箱内无需拧开瓶盖；可旋转切换密封档位，用于样本转运。
3. 人体工学斜颈大口，移液器、细胞刮可以完整触及瓶底生长面；瓶身模刻 + 印刷双重体积刻度，方便培养基定量。
4. 扁平化瓶身，堆叠节省孵箱空间；高透明聚苯乙烯，显微镜直接观察细胞汇合度；一次性使用，禁止高压灭菌。

下游应用

肿瘤细胞系、原代贴壁细胞传代扩增，细胞转染，药物毒性评价，部分悬浮细胞 CO₂孵箱培养。

储存条件

室温干燥避光存放，远离高温；一次性耗材，不可高压灭菌。

⚠️提示：孵箱内使用置于透气档位；样品转运切换至密封档位。

English Product Introduction

Product Description

Nunc™ EasYFlask™ 25 cm² cell‑culture flask (Cat.No.156367), canted‑neck design with 0.2 μm hydrophobic PTFE filter cap. Treated with Nunclon™ Δ plasma surface for cell attachment. Constructed from clear polystyrene, gamma‑irradiated sterile, non‑pyrogenic, non‑cytotoxic, animal‑component‑free and USP Class‑VI compliant. The filter enables sterile gas‑exchange while preventing microbial contamination for incubation inside CO₂ incubatorsSigma-Aldr....

• Catalog No.: 156367
• Growth area: 25 cm²
• Recommended working volume: 7 mL
• Pack size: 10 flasks per inner pack, total 200 per case
• Cap: HDPE filter cap; one‑third‑turn opening/closing. Visible “Y” marker to identify vent‑on / vent‑off status even stacked in incubator.

Key Features

1. Nunclon™ Δ surface: Plasma‑modified surface for reliable attachment, proliferation and differentiation of cell lines and primary cells, excellent lot‑to‑lot consistency.
2. 0.2 μm hydrophobic filter cap: Permits CO₂/O₂ exchange and blocks microbial contamination. Rotate cap to sealed position for sample transportation.
3. Ergonomic angled wide neck for full access to growth surface by pipettes and cell scrapers. Dual molded & printed volume graduations on flask wall.
4. Low‑profile stackable body to save incubator space. Clear polystyrene for direct microscopic observation. Single‑use, non‑autoclavable.

Applications

Adherent cell expansion, primary‑cell culture, cell transfection, cytotoxicity assay, suspension‑cell culture in CO₂ incubator.

Storage

Store at room temperature under dry and dark conditions. Do not autoclave.

Note: Keep cap at vent position for incubator culture; switch to sealed mode for transport.