相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 库存:
现货
- 保修期:
见包装
- 现货状态:
现货
- 供应商:
上海然其生物科技有限公司
- 规格:
8个/板/6板/箱
| 规格: | 8个/板 | 产品价格: | ¥302.3 |
|---|---|---|---|
| 规格: | 6板/箱 | 产品价格: | ¥1814.0 |
中文介绍
- 膜材质:PET(聚酯,透明)
- 膜直径:6.4mm(约 6.5mm)
- 孔径:8.0μm
- 孔密度:约 1×10⁵个 /cm²
- 生长面积:0.3cm²
- 适配板型:标准 24 孔培养板
- 包装:1 个 / 包,48 个 / 箱
- 灭菌:γ 射线辐照灭菌,无热源、无 DNase/RNase
- 蚀刻 PET 膜:表面光滑,圆柱形贯穿孔,低蛋白吸附,利于细胞观察与物质转运
- 悬挂式设计:稳定架于 24 孔板内,方便移液,支持细胞共培养、跨层生长模型
- 外壁无 TC 处理:避免细胞沿外壁非特异性生长,保证实验准确性
- 透明膜:可直接用相差显微镜观察上 / 下室细胞形态,无需揭膜
- 细胞侵袭实验(Transwell 侵袭)
- 细胞迁移、趋化性检测
- 肿瘤细胞转移研究
- 细胞共培养(如肿瘤 - 基质、免疫细胞互作)
- 跨膜转运、分泌因子检测
英文介绍
- Membrane:Transparent PET (Polyethylene terephthalate)
- Membrane Diameter:6.4 mm
- Pore Size:8.0 µm
- Pore Density:~1 × 10⁵ pores/cm²
- Growth Area:0.3 cm²
- Plate Compatibility:Standard 24-well plates
- Pack:1/pack, 48/case
- Sterilization:Gamma irradiated, pyrogen-free, DNase/RNase-free
- Track-etched PET membrane with smooth surface and cylindrical through-pores; low protein binding.
- Hanging design fits securely in 24-well plates, enabling easy pipetting and co-culture systems.
- Non-TC-treated housing prevents cell overgrowth on insert walls.
- Transparent membrane allows direct microscopic observation of cells on both sides.
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验一、肿瘤侵袭 / 转移(最常用)
- Albini A, et al. (2016). Extracellular Matrix Invasion in Metastases and Angiogenesis: Commentary on the Matrigel “Chemoinvasion Assay”. Cancer Res, 76(16): 4595-4597.
引用:Cell invasion was assessed using Falcon 24-well inserts (8.0 μm, Cat. No. 353097, Corning) coated with Matrigel.
- Liu Y, et al. (2022). LncRNA XIST promotes breast cancer metastasis by sponging miR-34a to upregulate SIRT1. Cell Death Dis, 13: 421.
方法:Transwell assays were performed with Corning Falcon 353097 inserts (8 μm).
- Jin J, et al. (2019). Tunneling Nanotubes Mediated microRNA-155 Intercellular Transportation Promotes Bladder Cancer Cells' Invasive Capacity. Int J Nanomedicine, 14: 6769-6782.
引用:24-well Transwell chambers (8.0 μm, Falcon 353097) were used for invasion assays.
二、细胞迁移 / 趋化(无基质胶)
- Van den Steen P, et al. (2018). Chemokine-driven migration of neutrophils: Assays and quantification. Methods Mol Biol, 1751: 123-136.
引用:Falcon 353097 (8 μm PET) inserts were used in chemotaxis assays康宁公司.
- Kim H, et al. (2021). VEGF promotes endothelial cell migration through ... Angiogenesis, 24: 667-682.
方法:Migration was measured using Corning 353097 Transwell inserts (8.0 μm).
三、细胞共培养(非接触共培养)
- Zhang L, et al. (2020). Tumor-associated macrophages promote colorectal cancer stemness via IL-6/STAT3 signaling. J Exp Clin Cancer Res, 39: 215.
引用:Co-culture was performed using Falcon 353097 (24-well, 8 μm) inserts.
- Wang Y, et al. (2023). Endothelial cells regulate osteoblast apoptosis via exosomal miR-21. Int J Biol Sci, 19(8): 2345-2358.
方法:Cells were co-cultured in Corning 353097 Transwell systems.
abs7279 细胞小室(PC膜,6.5mm,孔径8.0um,含24孔板) 4块/箱 abs7270 细胞小室(PET膜,24mm,孔径0.4um,含6孔板) 4块/箱 abs7271 细胞小室(PET膜,24mm,孔径8.0um,含6孔板) 4块/箱 abs7274 细胞小室(PET膜,12mm,孔径0.4um,含12孔板) 4块/箱 abs7393 细胞小室(PET膜,12mm,孔径0.4um,含12孔板) 4块/箱 abs7275 细胞小室(PET膜,12mm,孔径
的研究。 下面参考guxuefeng战友和cosmosci战友的帖子具体来谈谈孔径的选择,当然不同细胞其体积不同,具体选择时要考虑到细胞大小。这里主要谈几种大家常用的实验: (1)共培养体系: 小于3.0 um孔径条件下,细胞不会迁徙通过,因此,若研究不涉及细胞运动能力,不需要细胞穿过聚碳酸酯膜,则应选择3.0μm以下孔径。常用0.4、3.0μm。我们实验室用的是0.4μm。 将细胞A种于上室,细胞B种于下室,可以研究细胞B分泌或代谢产生的物质对细胞A的影响。(2).趋化性实验
在 Dulbecco's ModifiedEagle's 培养基生长(DMEM)(Biochrom, Germany)含 10% 胎牛血清(FCS Gold, PAALaboratories, Germany)和 1% 青霉素/链霉素(Gibco Life Technologies, Germany)。 实验中,A549 细胞种在 BD Falcon(PET 膜,4.2 cm2, 孔径 0.4 μm, 孔密度 2.0 ± 0.2 × 106, BDFalcon, Germany)或者 Corning
技术资料暂无技术资料 索取技术资料










