Qiagen（凯杰）12362 去内毒素质粒大提试剂盒

EndoFree Plasmid Maxi Kit 去内毒素质粒大提试剂盒｜货号：12362（10 次）QIAGEN

中文产品介绍

产品名称：EndoFree Plasmid Maxi Kit 无内毒素质粒大量提取试剂盒 货号：12362 规格：10 次提取

产品简介

EndoFree Plasmid Maxi Kit 采用QIAGEN‑tip 500 重力流阴离子交换柱，搭配 QIAfilter Maxi 过滤柱，无需高速离心即可澄清碱裂解液；内置 Buffer ER 去除革兰氏阴性菌来源内毒素。推荐菌液起始量 100‑250 mL 大肠杆菌 LB 过夜培养物；单次最高产量可达10 mg 高拷贝质粒 / 柯斯质粒 DNA；最终 DNA 内毒素含量＜0.1 EU/μg，纯度优于两次氯化铯（CsCl）密度梯度离心。全部缓冲液为无内毒素级别；附带 LyseBlue 裂解指示剂，可视化监控裂解‑中和反应，避免基因组 DNA 污染。适用于对内毒素高度敏感的原代细胞转染、动物体内注射等严苛实验。

注意：为重力流纯化体系，非离心柱试剂盒；Buffer ER 新版配方已去除 Triton X‑114，符合欧盟 REACH 法规要求QIAGEN。

主要特点

1. 极低内毒素水平：内毒素＜0.1 EU/μg，适合原代细胞、干细胞转染、体内动物实验。
2. QIAfilter 快速澄清裂解液：过滤柱直接去除菌体碎片，无需长时间高速离心，节省操作时间。
3. 高产量高纯度：单次最高 10 mg 质粒 DNA；阴离子交换去除 RNA、蛋白、基因组 DNA、脂多糖杂质。
4. LyseBlue 裂解指示剂：直观判断裂解与中和终点，降低操作失误，减少质粒损失。
5. 无有毒试剂：不使用氯化铯、溴化乙锭、苯=酚‑氯仿，操作安全。
6. 无内毒素级全套缓冲液，从源头避免外源内毒素引入。

下游应用

原代细胞 / 干细胞转染、动物体内注射实验、RNA 病毒载体构建、体外转录、CRISPR 载体、基因治疗前期体外评估、高灵敏度分子克隆。

试剂盒组分（10 次）

QIAGEN‑tip 500 纯化柱 ×10；QIAfilter Maxi 过滤柱 ×10；Buffer P1（含 RNase A）；Buffer P2；Buffer P3；Buffer QBT；Buffer QC；Buffer ER 内毒素去除缓冲液；Buffer QF 洗脱缓冲液；LyseBlue 指示剂。

储存条件

tip 柱、过滤柱及全部缓冲液室温干燥避光保存（15‑25 ℃）；RNase A 加入 Buffer P1 后，Buffer P1 需 2‑8 ℃保存，可稳定 6 个月；其余缓冲液室温稳定 2 年；缓冲液出现盐析出，37 ℃水浴溶解后再使用。

仅供科研使用，不可用于临床诊断、预防或治疗用途。

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English Product Introduction

Product Name: EndoFree Plasmid Maxi Kit Cat.No.: 12362 Size: 10 preps

Product Description

The EndoFree Plasmid Maxi Kit utilizes gravity‑flow anion‑exchange QIAGEN‑tip 500 columns together with QIAfilter Maxi cartridges for lysate clarification without high‑speed centrifugation. The integrated Buffer ER step removes bacterial endotoxin. Recommended starting culture volume: 100‑250 mL E. coli overnight LB culture. Yield up to 10 mg high‑copy plasmid or cosmid DNA, with final endotoxin level ＜0.1 EU/μg, purity exceeding double CsCl density‑gradient centrifugation. All buffers are endotoxin‑free grade. Supplied LyseBlue reagent visualizes alkaline lysis‑neutralization reaction to minimize genomic‑DNA contamination. Ideal for endotoxin‑sensitive applications such as primary‑cell transfection and in‑vivo animal injection experiments.

Note: Gravity‑flow tip‑based system, not spin‑column kit. New‑formula Buffer ER is Triton X‑114‑free and REACH‑compliantQIAGEN.

Key Features

1. Ultra‑low endotoxin: ＜0.1 EU/μg, well‑suited for primary cells, stem‑cell transfection and in‑vivo animal studies.
2. QIAfilter cartridge clarifies lysate directly, eliminating lengthy high‑speed centrifugation.
3. High yield and high purity: up to 10 mg plasmid DNA per prep; anion‑exchange removes RNA, protein, genomic DNA and lipopolysaccharide contaminants.
4. LyseBlue lysis indicator visualizes lysis‑neutralization end‑point to reduce handling‑induced DNA loss.
5. Hazardous‑reagent‑free workflow: no CsCl, ethidium bromide or phenol‑chloroform.
6. Complete set of endotoxin‑free buffers to prevent exogenous endotoxin contamination.

Downstream Applications

Primary / stem‑cell transfection, in‑vivo animal injection, viral vector construction, in‑vitro transcription, CRISPR vector preparation, pre‑clinical in‑vitro gene‑therapy evaluation, demanding molecular cloning.

Kit Contents (10 preps)

QIAGEN‑tip 500 tips ×10; QIAfilter Maxi cartridges ×10; Buffer P1 (with RNase A); Buffer P2; Buffer P3; Buffer QBT; Buffer QC; Buffer ER; Buffer QF; LyseBlue reagent.

Storage

Store tips, cartridges and all buffers dry at room temperature (15‑25 °C). After adding RNase A to Buffer P1, store Buffer P1 at 2‑8 °C (stable for 6 months). Remaining buffers stable for 2 years at room temperature. Dissolve salt precipitates at 37 °C before use.

For research use only. Not intended for diagnosis, prevention or treatment of human or animal diseases.