FGF-21/FGFL, Human, Recombinan

FGF-21/FGFL, Human, RecombinantFGF-21/FGFL, Human, RecombinantFibroblast Growth Factor 21;FGF-21;FGF21

FGF-21/FGFL, Human, Recombinant PCK132

背景介绍：Fibroblast Growth Factor 21 (FGF21) is a growth factor that belongs to the FGF family. FGF family proteins play a central role during prenatal development and postnatal growth and regeneration of mamy tissues, by promoting cellular proliferation and differentiation. FGF21 is a potent activator of glucose uptake on adipocytes, protects animal from diet-induced obesity when overexpression in transgenic mice, and lower blood glucose and triglyceride levels when therapeutically adiministered to diabetic redents. FGF21 is produced by hepatocytes in reponse to free fatty acid stimulation of a PPARa/RXR dimeric complex. This situation occurs clinically during starvation, or following the ingestionof a highly-fat/low-carbohydrate diet.Upon FGF21 secretion, white adipose tissue is induced to release free fatty acids from triglyceride stores. Once free fatty acid reach hepatocytes, they are oxidized and reduced to acetyl-CoA. The acetyl-CoA is recombined into 4-carbon ketone bodies, release, and transported to peripheral tissue for TCA processing and energy generation.

产品形态	粉末
产品规格	1mg、10μg、50μg、500μg
别名	Fibroblast Growth Factor 21;FGF-21;FGF21
物种	人
表达宿主	E.coli
序列信息	His29-Ser209
检索号	AAH18404.1
分子量	21.69 KDa
表观分子量	23 KDa
标签	6His tag at the N-terminus
纯度	> 95% as determined by reducing SDS-PAGE.
内毒素	< 0.1 EU/μg
储存条件	Lyophilized protein should be stored at -5~-20℃, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at -5~-20℃ for 3 months.
运输条件	Ambient temperature or ice pack.
有效期	12个月
制剂	Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 100 mM NaCl, 2 mM EDTA, pH 9.0.
复融	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in sterile water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
体系描述	GMP生产和质量体系 公司符合cGMP的要求，已通过NMPA的第一类医疗器械生产备案，且通过ISO 13485和ISO 9001标准认证。公司生产的FGF-21/FGFL, Human, Recombinant符合ISO 9001的cGMP标准。

产品问答FAQs：

Q：收到细胞因子产品用什么溶剂复融？

A：1、细胞因子产品大部分为冻干形式发货，少部分液体形式发货； 2、冻干粉状态的因子产品需指定溶剂复溶，请参考产品手册【复融】方法，按照要求使用对应溶剂溶解，大部分因子为无菌水复融，少部分需要无菌水以外的特殊溶剂溶解的产品则随货会赠送相应的复融试剂；冻干粉溶解后如您需要进一步稀释分装，需使用含载体蛋白(如0.1% BSA，10%FBS，5%HSA 或 5% 海藻糖)的溶液进一步稀释后分装冷冻保存。 3、液体形式因子产品不需要复溶，具体浓度详见产品标签，按照实验需要用含载体蛋白(如0.1% BSA，10%FBS，5%HSA 或 5% 海藻糖)的溶液进一步稀释后分装冷冻保存即可；

Q：因子到货了，里面有两管溶液，一个是水，一个是海藻糖溶液，用哪一个稀释呢？

A：1、将装有细胞因子的管子放入小离心机内短暂离心20~30s（离心机最大转速即可），使附着在管壁的微量蛋白聚集在管底； 2、先用无菌水溶解因子，很少量水即可溶解因子，注意计算溶解后浓度不低于100 μg/mL； 3、用赠送的含海藻糖的PBS溶液进一步稀释到您实验合适的浓度即可，然后分装后放入-20℃以下保藏； 4、因子复融重悬过程应轻柔，不可以震荡涡旋。

Q：细胞因子离心前需要放置室温吗？

A：不用提前放置室温.

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FGF-21/FGFL, Human, Recombinant Ambient temperature or ice pack. Lyophilized protein should be stored at -5~-20℃, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at -5~-20℃ for 3 months. 12个月